Identification and Basic Application Research of Two Abiogenous Azotobacter

doi:10.7668/hbnxb.2018.04.030

Abstract

Abstract: In order to identify two strains of azotobacter isolated from coastal saline soil, explore the environmental adaptability of two strains and inoculated effect by pot experiment in wheat. Morphological observation, physiological and biochemical determination and 16S rDNA sequence identification were carried out. We also studied environmental adaptability of bacteria by the analysis of IAA and effect of wheat seedlings was evaluated by pot experiment in wheat.The characteristic of morphology and 16S rDNA sequence analysis revealed that strain 1 was classified as Variovorax paradoxus. And strain 2 was identified as Azospirillum zeae. The nitrogenase activity of strain 1 and strain 2 were 4.71 and 2.84 μmol/ (h·mL). The analysis of IAA indicated that both of two strains had strong ability to keep themselves under acid or alkalinity. The two strains had strong ability of IAA under 5% concentration of NaCl concentration. The strain 1 utilized mannitol most and the IAA amount of strain 2 was significantly higher under glucose. The results of strains secreted IAA under exogenous NH₄⁺ showed that low concentration of NH₄⁺ stimulate the strain 1 to secrete IAA.When the concentration of NH₄⁺ was over 0.5 mmol/L, IAA was inhibited in different degrees. The curve of strain 2 showed a M curve. Pot experiments showed that strain 1 and strain 2 significantly increased height and dry weight of wheat shoots, promoted the absorption of nitrogen, phosphorus and potassium in wheat, and improved the content of available nutrients in soil.The soil urease activity of two strains increased by 16.00% and 48.15%, and the strain 2 had the best effect. The two strains have high nitrogenase activity and strong adaptability. The treatments of two strains have good effect on the growth of wheat. The results can be used for the cultivation of new low yield saline alkali soil and provide technical support for optimizing the fertilization structure of medium and high yield saline alkali farmland.

Key words: Coastal saline soil, Abiogenous azotobacter, Nitrogenase activity, Auxin, Soil urease, 16S rDNA

CLC Number:

S154.1

WANG Huiqiao, CHEN Weifeng, ZHUGE Yuping, HU Guoqing, YU Haiyan, FU Naixin, DONG Yuanjie. Identification and Basic Application Research of Two Abiogenous Azotobacter[J]. Acta Agriculturae Boreali-Sinica, 2018, 33(4): 210-216. doi: 10.7668/hbnxb.2018.04.030.

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