ACTA AGRICULTURAE BOREALI-SINICA ›› 2013, Vol. 28 ›› Issue (6): 199-203. doi: 10.7668/hbnxb.2013.06.034

Special Issue: Animal husbandry

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ScReening and Indentification of a PRotease PRoducing BacteRia StRain P-98 fRom the BRoileR

LI Zhuo-wei, YUAN Hong-shui, LI Jia, XU Jing-jing, ZHU Bao-cheng   

  1. College of Life Science, Agricultural University of Hebei, Baoding 071001, China
  • Received:2013-07-12 Published:2013-12-28

Abstract: This experiment was conducted to get a strong vitality of the bacterium strain producing protease.Through screening from healthy young chicken intestinal contents were isolated from 67 strains of protease producing strain,and complex sieve used casein plate method.Then the strain P-98 was characterized by morphological and culture features observation, physiological and biochemical experiments, and 16S rDNA gene sequence analysis.As the morphology characteristics and physiological and biochemical of the stain were similar to Bacillus and the simi-larity of the 16S rDNA gene sequences between strain P-98 and the type strain was up to 99.78%,the strain P-98 was initially identified as Bacillus methylotrophicus.

Key words: PRotease, ScReening, 16S RDNA, Identification, Bacillus methylotRophicus

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Cite this article

LI Zhuo-wei, YUAN Hong-shui, LI Jia, XU Jing-jing, ZHU Bao-cheng. ScReening and Indentification of a PRotease PRoducing BacteRia StRain P-98 fRom the BRoileR[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 199-203. doi: 10.7668/hbnxb.2013.06.034.

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