Screening,Identification and Activity Analysis of Antagonistic Bacteria Against Fusarium Wilt of Watermelon from Chrysanthemum coronarium rhizosphere

doi:10.7668/hbnxb.2016.S1.076

Abstract

Abstract: To investigate the roles and effects of Chrysanthemum coronarium in disease controlling on watermelon wilt induced by Fusarium oxysporum f.sp.Niveum (FON).An antagonistic bacterium was isolated from root exudate of Chrysanthemum coronarium rhizosphere.This antagonistic bacterium exhibited significantly ability to watermelon wilt pathogen Fusarium oxysporum f. sp. Niveum (FON) based on the plate confrontation experiment.The TK-3 bacterial strains could antagonize Fusarium oxysporum f. sp. Niveum (FON).In a pot-test, Fusarium wilt of watermelon were reduced by TK-3 strain treated in watermelon were 48.7% of control in 30 days.The results of morphological,physiological and biochemical characterization,and 16S rDNA-based molecular identification showed that the isolated antagonistic bacterium was Bacillus amylolique-faciens.Production of antibacterial metabolites experiments showed strain TK-3 had the ability of IAA production,siderophore production,NH₃ production,proteolytic activity and phosphate solubilization,but could not produce HCN.Results indicated that strain TK-3 had a great potential on bio-control application.

Key words: Antagonistic bacteria, Fusarium wilt of watermelon, 16S rDNA identification, Antifungal metabolites, Bacillus amylolique-faciens

CLC Number:

S436.42
S154

KANG Jinlei, HAN Xu, ZHANG Shiqi, WANG Bingqing, YANG Wenjie. Screening,Identification and Activity Analysis of Antagonistic Bacteria Against Fusarium Wilt of Watermelon from Chrysanthemum coronarium rhizosphere[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 447-452. doi: 10.7668/hbnxb.2016.S1.076.

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