Screening and Identification of Antagonistic Bacterium against Colletoichum wilt

doi:10.3969/j.issn.1000-7091.2012.02.042

Abstract

Abstract: In order to obtain antagonistic bacteria against Fusarium oxysporium，237 bacillus strains were isolated and screened from soil samples of different districts． A bacillus strain 3A3-15 collected from cotton soil of Raoyang County in Hengshui was obtained via preliminary screening and secondary screening． It had strong and broadspectrum inhibition capability． We characterized the strain 3A3-15 by morphological and culture features observation，physiological and biochemical experiments，and 16S rDNA sequence analysis． As the similarity of the 16S rDNA sequences between strain 3A3-15 and the type strain CR-502 was up to 99． 92%，the strain 3A3-15 was finally?identified as Bacillus velezensis．

Key words: Fusarium oxysporium, Antagonistic bacteria, Screening, IdentificationIdentification, Bacillus China velezensis

CLC Number:

S432.4+2

LIAN Cai, GUO Xiao-jun, ZHU Bao-cheng, WANG Jian-ming, ZHANG Chao, CAO Xiao-lu, YAO Na. Screening and Identification of Antagonistic Bacterium against Colletoichum wilt[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 222-225. doi: 10.3969/j.issn.1000-7091.2012.02.042.

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