Effect of Rhizobium Inoculation on Nitrogen Fixation Activity and Biomass of Medicago sativa L.

doi:10.7668/hbnxb.2016.04.033

Abstract

Abstract: To select the suitable high efficient nodule bacteria in the ecological region of Shanxi to the main popularized varieties,make full use of the symbiotic nitrogen fixation of alfalfa,and to establish a high efficient,high yield,high quality alfalfa production system,nine strains were screened from the center of Microbial Resource and Application of CAAS.Soil from Datong of Shanxi Province to screen highly-effective strains for Magnum-salt (a cultivar of Medicago sativa) in pot in greenhouse was used in this study.The number of nodules,activity of nitrogen fixing enzyme,plant height and biomass were used as test indexes.Strains of CCBAU30015,CCBAU NMC024-1,HBU07004 and CCBAU30198 were selected by this way.Experiments were conducted in field to test their inoculating responses.The results showed that compared with the uninoculated,the hay yield of M.sativa was increased 18.7%,15.8%,11.3% and 13.3% after inoculating with the strains of CCBAU30015,CCBAU NMC024-1,HBU07004 and CCBAU30198 respectively.And the yield of crude protein (CP) was increased 10.9%,10.2%,9.7% and 10.1%,accordingly.Among the most prominent performance of CCBAU30015,the differences were significant (P<0.05).These results indicated that highly-effective Sinorhizobium meliloti screened from soil and M.sativa cultivar could be applied in production.

Key words: Rhizobium, Medicago sativa L., Screening, Hay yield

CLC Number:

S551
Q939

HAN Zhishun, ZHENG Minna, LIANG Xiuzhi. Effect of Rhizobium Inoculation on Nitrogen Fixation Activity and Biomass of Medicago sativa L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(4): 214-219. doi: 10.7668/hbnxb.2016.04.033.

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References

[1] 孙启忠,玉柱,赵淑芬.紫花苜蓿栽培利用关键技术[M].北京:中国农业出版社,2008.
[2] 朱秋莲,邢肖毅,程曼,等.宁南山区典型植物根际与非根际土壤碳、氮形态[J].应用生态学报,2013,24(4):983-988.
[3] 曾昭海,隋新华,胡跃高,等.紫花苜蓿-根瘤菌高效共生体筛选及田间作用效果[J].草业学报,2004,13(5):95-100.
[4] Provorov N A,Saimnazarov U B,Tanriverdiev T A,et al.The contributions of plant and bacteria genotypes in the growth and Nitrogen accumulation of inoculated alfalfa[J].Plant and Soil,1994,164(2):213-219.
[5] 曾昭海,胡跃高,陈文新,等.共生固氮在农牧业上的作用及影响因素研究进展[J].中国生态农业学报,2006,14(4):21-24.
[6] 宁国赞,刘惠琴,马晓彤,等.中国苜蓿根瘤菌大面积应用研究现状及展望[A].首届中国苜蓿发展大会论文集[C],北京:中国草原学会,2001.
[7] Peterson T A,Russelle M P.Alfalfa and the nitrogen cycle in the corn blet[J].Journal of Soil and Water Conservation,1991,3:229-235.
[8] 马霞,王丽丽,李卫军,等.不同施氮水平下接种根瘤菌对苜蓿固效能及种子生产的影响[J].草业学报,2013,22(1):95-102.
[9] 陈丹明,曾昭海,隋新华,等.紫花苜蓿高效共生根瘤菌的筛选[J].草业科学,2002,19(6):27-31.
[10] 陈文新.豆科植物根瘤菌-固氮体系在西部大开发中的作用[J].草地学报,2004,12(1):1-2.
[11] 石茂玲,邓波,刘忠宽,等.5株根瘤菌接种紫花苜蓿的效果[J].草业科学,2015,32(1):104-106.
[12] Tan G Y,Tan W K.Net assimilation rate and relative Nitrogen assimilation rate in relation to the dry matter production of alfalfa cultivars[J].Plant and Soil,1981,59(2):185-192.
[13] 刘晓云,郭振国,李乔仙,等.南苜蓿高效共生根瘤菌土壤的筛选[J].生态学报,2011,31(14):4034-4041.
[14] 黄新,王亚琴,刘建新,等.接种根瘤菌对不同紫花苜蓿品种结瘤和生物学产量的影响[J].浙江农业学报,2005,17(6):391-394.
[15] 房增国,赵秀芬,孙建好,等.接种根瘤菌对蚕豆/玉米间作系统氮营养的影响[J].华北农学报,2009,24(4):124-128.
[16] 肖猛,刘晓云,刘桂霞,等.BOX-PCR分子标记对补播紫花苜蓿共生根瘤菌田间竞争结瘤能力的研究[J].华北农学报,2011,26(1):187-191.
[17] 钟文文,张小平,Lindstrom K.高效苜蓿根瘤菌的筛选[J].西北农业学报,2006,15(3):69-74.
[18] 王平,周道玮,姜世成.半干旱地区禾豆混播草地生物固氮作用研究[J].草业学报,2010,19(6):276-280.
[19] Bosworth A H,Williams M K,Albrecht K A,et al.Alfalfa yield response to inoculation with recombinant strains of Rhizobium meliloti with an extra copy of dctABD and/or modified nifA expression[J].Applied and Environmental Microbiology,1994,60(10):3815-3832.
[20] 曹宏,章会玲,盖琼辉.22个紫花苜蓿品种的引种试验和生产性能综合评价[J].草业学报,2011,20(6):219-229.

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