Effect of in vitro Mutagenesis with Pingyangmycin and NaCl-directed Screening on Production and Quality of Offspring in Peanut

doi:10.7668/hbnxb.2015.01.034

Abstract

Abstract: To expand the salt-tolerant gene resources of peanut, we conducted in vitro mutagenesis with pingyangmycin(PYM)as the mutagen and directed screening on a medium containing NaCl in the previous experiment.In this study, pods of M₃ individual plants from those original NaCl-tolerant plants were collected and weighted after harvest and sun drying and the results indicated that 5 original HYP-tolerant, regenerated plants produced≥3 offspring with over 60 g/pods per plant.M₄ seeds were tested for quality by Near Infrared Spectroscopy, and the results indicated that there was substantial variation in the protein content, oleic acid, linoleic acid content, and oil content, including seven with higher protein content(>30%)and 11 individuals with higher oil content(>55%).According to the results of pod weight per plant and quality traits combined with a germination test with a 0.7% NaCl solution of M₄ seeds, we screened a few individuals with high yield, oil content and salt tolerance.We concluded that the use of PYM-based in vitro mutagenesis in combination with directed screening with NaCl and Near Infrared Spectroscopy technology is a new effective pathway for breeding of peanut.

Key words: Peanut, Pingyangmycin(PYM), Directed screening, Near infrared spectroscopy technology, Quality

CLC Number:

S565.01

WANG Ya, QIAO Li-xian, WU Xiu-ling, HU Xiao-hui, WANG Jing-shan, SUI Jiong-ming. Effect of in vitro Mutagenesis with Pingyangmycin and NaCl-directed Screening on Production and Quality of Offspring in Peanut[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 202-206. doi: 10.7668/hbnxb.2015.01.034.

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