Tissue Specificity Strong Promoter Screening of Poplar

doi:10.7668/hbnxb.2014.S1.009

Abstract

Abstract: In order to screen the xylem tissue specific and strong function promoter,three plant expression vector pMDCesAP-GUS,pJCesAP-GUS,pDMDCesAP-GUS,fused with GUS report gene and MDCesA promoter,JCesAP promoter,DMDCesAP promoter (including two series MDCesAP promoter) acquired earlier,are transformed to poplar by agrobacterium mediated transformation.With qualitative and quantitative fluorescence detection of GUS activity,the three promoter in transgenic plants expressing ability and level are detected.Fusion of the screened xylem specific promoter and insect-resistant gene will improve the poisonous protein expression in the xylem of the quantity,which is of great significance for prevention and control of boring beetles.

Key words: Xylem specific promoter, Screening, GUS reporter gene, Fluorescence quantitative detection

CLC Number:

LI Jie, ZHAO Jin-feng, ZHANG Shuang, DU Ke-jiu. Tissue Specificity Strong Promoter Screening of Poplar[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(S1): 40-43. doi: 10.7668/hbnxb.2014.S1.009.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2014.S1.009

http://www.hbnxb.net/EN/Y2014/V29/IS1/40

[1]	MENG Yaning, YAN Libin, ZHANG Hongxiao, ZHAO Haitao, FAN Yanqin. *Screening and Detection of Resistance to Phytophthora* Blight Gene in Pepper Using Molecular Markers** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 338-341.
[2]	WU Shanshan, XU Xuexin, ZHANG Xia, GE Hongmei, ZHAO Changxing, WANG Weihua, SHI Yan, ZHAN Yuan. Relationship Analysis of Chlorophyll Fluorescence Parameters and Drought Resistance in Different Winter Wheat Varieties at Seedling Stage [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 90-99.
[3]	ZHOU Dahu, QU Ziyang, NIE Liyun, LIU Jialong, FU Junru, TANG Wenbang, HE Haohua. Analysis of Low Nitrogen Tolerance Characteristics of High Quality Local Variety Ehuxiangdao [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(5): 132-139.
[4]	ZHANG Yingjun, GAO Huimin, LI Ziqian, HU Mengyun, SUN Lijing, LIU Qian, Lü Liangjie, LI Hui. *Screening of Fhb1* Germplasms Resistant to Fusarium Head Blight and Its Putative Ancestor in North of Huang and Huai Winter Wheat Region** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(2): 196-202.
[5]	YANG Yanyan, QIAO Songlin, LI Rui, GUO Junqing, LI Qingmei, TENG Man, WANG Li, ZHAO Dong, LI Xuewu, DENG Ruiguang, ZHANG Gaiping. Development of a IPMA Method Applicable for Screening mAb Targeting PRRSV [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 217-223.
[6]	JIANG Qi, CHEN Zhiwei, LIU Chenghong, HE Ting, GUO Guimei, GAO Runhong, XU Hongwei, LI Yingbo, LU Ruiju, HUANG Jianhua. Screening and Identification Indices of Low-nitrogen Tolerance for Barley Landraces at Seedling Stage [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 148-155.
[7]	LIANG Fenghao, LANG Lina, LIU Yaping, WANG Jing, ZHANG He, XU Aixia, HUANG Zhen. *Resource Screening and Germplasm Creation of Brassica napus* Resistant to Clubroot** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 219-225.
[8]	TENG Yuxi, ZHANG Hongyan, XI Wendi, LIANG Shiyu, FANG Yulin, WANG Hua, JIN Guojie. Screening of Bio-control Bacteria Against Grape Mycosis Based on the Method with Substrate Stress [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(S1): 253-258.
[9]	XING Fangfang, GAO Mingfu, ZHUO Youyou, HU Zhaoping, LI Xinzhu. *Screening and Identification of Phosphate Solubilizing Bacteria in Hordeum vulgare* Rhizosphere and Its Growth Promoting Effect** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 252-257.
[10]	SHAO Yun, HAO Zhenzhen, WANG Hailei, LI Xiangli, WANG Wenfei, CHEN Jingwen, WANG Jingruo. Screening of Lead Resistance Strains and Their Absorption Characteristics in Wheat Field Soil [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(6): 199-205.
[11]	HAN Zhishun, ZHENG Minna, LIANG Xiuzhi. *Effect of Rhizobium* Inoculation on Nitrogen Fixation Activity and Biomass of Medicago sativa L.** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(4): 214-219.
[12]	ZHANG Yu-mei, LI Han-bing, BI Ming-zhao, WANG Zhi, ZHANG Na, YANG Wen-xiang, LIU Da-qun. Screening of Wheat Endophytic Bacteria W-1 and the Inhibition to Rhizoctonia cerealis [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 278-282.
[13]	ZHENG Jin-hui. Selection and Mutation Breeding of Alkaline Protease-producing Strains [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(5): 122-124.
[14]	WANG Ya, QIAO Li-xian, WU Xiu-ling, HU Xiao-hui, WANG Jing-shan, SUI Jiong-ming. *Effect of in vitro* Mutagenesis with Pingyangmycin and NaCl-directed Screening on Production and Quality of Offspring in Peanut** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 202-206.
[15]	LI Ang, WU Zhi-ming, ZHOU Zhi-lin, ZHAO Dong-lan, ZHANG An, MA Dai-fu, LI Ya-dong, TANG Jun, CAO Qing-he. The Construction of Genomic Fosmid Library and Library PCR Screening System on Ipomoea trifida(Kunth) G. Don [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(2): 45-50.

Please choose a citation manager

Content to export