Construction of Mouse Dazl Eukaryotic Expression Vector and Expression

doi:10.7668/hbnxb.2014.S1.008

Abstract

Abstract: To study the function of Dazl gene and to confirm its promotion role during the differentiation of stem cells to germ cells,the full coding sequence of Dazl gene was cloned from the ovaries of female mice of 13.5 days post coitum (dpc).Then it was ligated into eukaryotic expression vector pcDNA3.1 after digested by the restriction endonuclease Hind Ⅲ and Kpn Ⅰ.The mouse fibroblasts were transfected using the linearized pcDNA3.1-Dazl.The results of qRT-PCR and Western Blot showed the foreign gene Dazl had transfected into eukaryotic cells.The study would provide the foundations for the induction and differentiation of stem cells to germ cells.

Key words: Dazl, Eukaryotic expression vector, Transfection

CLC Number:

SUN Xing-hong, SUN Xiao-feng, SUN Lei-lei, LI Lan. Construction of Mouse Dazl Eukaryotic Expression Vector and Expression[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(S1): 35-39. doi: 10.7668/hbnxb.2014.S1.008.

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