Construction of Eukaryotic Expressing Plasmid of Chicken CT Gene and Its Expression in COS-1

doi:10.7668/hbnxb.2008.S1.004

Abstract

Abstract: Chicken calcitoni gene was amplified from the recombinant plasmid named pGEM-T-CT by digested (Hind Ⅲ + Bam HI)and cloned into pCDNA3.The recombinant plasmid was transfected into COS-1 with PEI.72 hafter transfection, the expression of CT were successfully tested by indirectimmunofluorescence assay (IFA)with the antiserumof CT expressed in COS-1.

Key words: Calcitoni, Transfection, IFA

CLC Number:

YU Lu, WANG Ya-qian, ZHANG Shi-yuan, BU Xiang-bin, LI Zhi-xue. Construction of Eukaryotic Expressing Plasmid of Chicken CT Gene and Its Expression in COS-1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S1): 15-18. doi: 10.7668/hbnxb.2008.S1.004.

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