Abstract:
To establish the mouse IgGFc Receptor(moFcγRII)stable expression cell line.The coding sequence of FcγRII was amplified and cloned into the mammalian expression vector pcDNA3.0 under control of the CMV promoter.The COS-7 cells were tranfected with the recombinant plasmids,and the FcγRII stable expression COS-7 cells were selected by the antibiotics of G418,and subcloned by well-known method of limiting dilution.Rosetting test of the binding of mouse IgG-sensitized chicken erythrocytes show that the percent of positive cells were more than 90%.The establishment of the mouse FcγRII stable expression cell line laid the necessary foundation for the identification of the linear epitope of IgG binding on mouse FcγRII,and further investing the immunologic mechanism of Fc receptor of IgG.
Key words:
Mouse FcRII,
Cell line,
Rosetting test,
Stable transfection
CLC Number:
MIAO Xian-wei, XI Jun, LIU Xi, ZHANG Gai-ping, DENG Rui-guang, LI Qing-zhou, ZHANG Li-na, YOU Lei-ming, LIU Yun-chao. Establishment of Cell Line Stably Transfected with the Constructed Plasmid of Mus Musculus FcγRⅡ[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 133-135. doi: 10.7668/hbnxb.2010.02.026.