Acta Agriculturae Boreali-Sinica

ACTA AGRICULTURAE BOREALI-SINICA ›› 2010, Vol. 25 ›› Issue (2): 136-139. doi: 10.7668/hbnxb.2010.02.027

Special Issue: Oil crops；

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The Method and Improvement of Two Dimensional Electrophoresis for Leaf Protein of Peanuts(Arachis hypogaea)

SHAO Yuan-yuan1, LIU Zhan-ji2, WANG Long-long1, BI Yu-ping3   

  1. 1. College of Life Science, Shandong Normal University, Jinan 250014, China;
    2. Hi-Tech Research Centre, Shandong Academy of Agriculture Sciences, Jinan 250100, China
  • Received:2010-01-25 Published:2010-04-28

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Abstract: The two dimensional electrophoresis(2-DE)technology was used to demonstrate the protein profile of leaves in peanut cultivar Luhua14.High quality and repeatable maps were obtained on according to TCA/acetone precipitation method with some improvement,such as sample extraction and dissolution,the pH rang of the immobilized gradient strips(IPG),the protein quantity of sample-loading and SDS-PAGE.This method and improvement will be helpful to proteomic research in peanut.

Key words: Peanuts, The soluble p rotein, Two2D gel electrophoresis, SDS2PAGE

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Cite this article

SHAO Yuan-yuan, LIU Zhan-ji, WANG Long-long, BI Yu-ping,. The Method and Improvement of Two Dimensional Electrophoresis for Leaf Protein of Peanuts(Arachis hypogaea)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 136-139. doi: 10.7668/hbnxb.2010.02.027.

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