A Protocol for Rapid Isolation of Total RNA from Plant Tissues

doi:10.7668/hbnxb.2010.02.028

Abstract

Abstract: In order to check the quality of total RNA isolated by NaAc/ethanol precipitation,total RNA of radicle,young leaves,young stems,immature seeds from wheat and flowers from different plant genus were extracted and checked.The results showed that total RNA obtained had good integrity and high purity.And the expression profiles of four frequently used house-keeping genes(GAPDH,Actin,Rubisco,Ubiqutin)were analyzed by normalization with Tubulin gene.The results of the amplification showed that the transcripts of the selected house-keeping genes were different at different time points of wheat leaves inoculated by leaf rust pathogens,moreover,the copy numbers of each house-keeping gene were inconsistent in wheat leaves.PCR amplification shows that NaAc/ethanol precipitation can meet with demands of down stream experiment of common molecular biology and is an ideal method of plant total RNA extraction.

Key words: Plant, Extraction of total RNA, House-keeping genes, Wheat, RT-PCR

CLC Number:

LIU Fen, YU Xiu-mei, LIU Da-qun,. A Protocol for Rapid Isolation of Total RNA from Plant Tissues[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 140-144. doi: 10.7668/hbnxb.2010.02.028.

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http://www.hbnxb.net/EN/Y2010/V25/I2/140

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