Comparison of DNA Extraction Methods from Leaves of Juglans hopeiensis Hu

doi:10.7668/hbnxb.2010.02.029

Abstract

Abstract: Using the leaves of Juglans hopeiensis Hu(Luohantou,Nananzhuang,Yihe1,C12,J16,M3,H12,C15,M10,MB,C13,M9,A7)as the experimental materials,three extraction methods of genomic DNA including high-salt and low-pH(2%PVP40,3%PVP40),SDS(0.5%,1%,1.5%)and modified CTAB(2%,3%)were compared,in order to select the optimal DNA extraction method and establish base of molecular population genetics of Juglans hopeiensis.The DNA samples were tested by agarose gel electrophoresis and ultraviolet spectrophotometer.The results showed that several extraction methods were able to extract genomic DNA,but there were some differences in purity and quality.3% PVP40 high-salt and low-pH method was the optimal method by compairisng purity and quality of the DNA.

Key words: JuglanshopeiensisHu, Leaf, GenomicDNA, Extraction, Method

CLC Number:

S664.1

LEI Ling, WANG Hong-xia, ZHANG Zhi-hua, HE Fu-qiang, GAO Yi, ZHAO Shu-gang, SUN Hong-chuan. Comparison of DNA Extraction Methods from Leaves of Juglans hopeiensis Hu[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 145-148. doi: 10.7668/hbnxb.2010.02.029.

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