Sequence Analysis of the E Gene of JEV Isolate BSF.ZZ-1 and BSF.ZZ-3 Passaged in BHK-21 Cells

doi:10.7668/hbnxb.2013.06.012

Abstract

Abstract: In this paper, the genetic stability of E gene of JEV isolates BSF.ZZ-1 and BSF.ZZ-3 passaged in BHK-21 cells(Baby hamster kidney cell line ) was studied.The results showed that the E gene is becoming stable after 60 passages in BHK-21 cells.After serial sucultivation,stable amino acid(aa) mutation happened at the sites E21(F→L),E200(T→A),E244(G→E),E279(N→S) and E426(G→D) in isolate BSF.ZZ-1 and sites E244 (G→E),E255(F→L),E285(M→L),E368(L→S) and E97(N→S) in isolate BSF.ZZ-3.No mutations were ob-served at part of the virulence correlated aa sites, such as E107,E138,E176,E177 and E315,similar to those of vaccine strain SA14-14-2.However,mutations at the virulence correlated aa sites of E279(M→K→M)of BSF.ZZ-3 happened repeatedly.Whether these mutations are correlated with the virulence variation and adaptability of JEV to host cells needs to be further studied.

Key words: JEV, BSF.ZZ-1, BSF.ZZ-3, BHK-21 cell line, SeRial subcultivation, E gene, Sequence analysis

CLC Number:

S852.65

HU Bo, TENG Man, YU Le-le, LUO Jun, CHI Jia-qi, SU Jing-wei, CHAI Shu-jun, ZHANG Gai-ping. Sequence Analysis of the E Gene of JEV Isolate BSF.ZZ-1 and BSF.ZZ-3 Passaged in BHK-21 Cells[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 71-76. doi: 10.7668/hbnxb.2013.06.012.

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