Isolation,Identification and Genetic VaRiation of PoRcine CiRcoviRus Type 2 Isolate 2010AHCY

doi:10.7668/hbnxb.2013.06.013

Abstract

Abstract: The aim of this study was to obtain the porcine circovirus type 2(PCV2) isolate 2010AHCY and to identify its biological characteristics, and to analyze the 50%tissue culture infective dose ( TCID⁵⁰ ) and the whole genome of this isolate.The samples which were detected PCV 1 negative and PCV2 positive were inoculated and pas-saged on PK15 cells.The isolate was identified by PCR and confirmed by immunofluorescent assay ( IFA).The TCID⁵⁰ of 2010 AHCY isolate was determined and the whole genome was sequenced and its molecular variations was analyzed by DNAStar software.A PCV2 strain 2010 AHCY was successfully isolated from samples.The results showed that the genome length of 2010AHCY was 1 767 bp and the TCID⁵⁰ was 10_4.23.Comparison analysis of the complete genomic sequences of 2010 AHCY and other strains indicated that the homologies among these 11 strains were 93.2%-99.9%and the highest homology of 99.9%to the HM038030 PCV2d strain.The phylogenetic trees revealed that the isolate 2010 AHCY belonged to genotype PCV 2 d and had a close relationship with PCV 2 b geno-type.This study provide foundations for studying the molecular epidemiology, variation and prevention of PMWS in Eastern China.

Key words: PoRcine ciRcoviRus type 2( PCV2), Isolation, Identification, Genetic vaRiation

CLC Number:

S852.65

WANG Xiao-min, HE Kong-wang, WANG Dong-tian, ZHOU Zhong-tao, MAO Ai-hua, YU Zheng-yu, WANG Wei, NI Yan-xiu, WEN Li-bin, ZHANG Xue-han, GUO Rong-li, LI Bin. Isolation,Identification and Genetic VaRiation of PoRcine CiRcoviRus Type 2 Isolate 2010AHCY[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 77-81. doi: 10.7668/hbnxb.2013.06.013.

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http://www.hbnxb.net/EN/Y2013/V28/I6/77

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