ViRus-induced Gene Silencing in RoRippa indica HieRn

doi:10.7668/hbnxb.2013.06.011

Abstract

Abstract: Virus-induced gene silencing system of Rorippa indica Hiern was constructed, which will be used in functional analysis of R.indica genes.Cloroplasto alterados( CLA) gene fragments of cotton, soybean and Arabidop-sis were obtained by using RT-PCR.The cloned CLA fragments were constructed into tobacco rattle virus vector (pYY13).The constructed vectors were transformed into Agrobacterium strain GV3101,which was used to inoculate R.indica plants.All the infected plants showed photobleaching symptom with different degrees.RT-PCR of target genes further confirmed the knock-down of CLA.The established VIGS system will contribute the further study of R.indica genes in the future research.

Key words: ViRus induced gene silencing, RoRippa indica HieRn, CLA gene, Tobacco Rattle viRus

CLC Number:

S636.9

ZHANG Yi, XU Ke-dong, YANG Song, LI Hao, CAO Peng, WEI Sen, WEI Dan-dan, LI Cheng-wei. ViRus-induced Gene Silencing in RoRippa indica HieRn[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 65-70. doi: 10.7668/hbnxb.2013.06.011.

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http://www.hbnxb.net/EN/Y2013/V28/I6/65

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