Identification and Construction of the Recombinant Vector of Virus Induced Gene Silencing of Soybean Heterodera glycines Ichinohe

doi:10.3969/j.issn.1000-7091.2012.03.043

Abstract

Abstract: In this research，the partial cDNA of Heterodera glycines Ichinohe troponin C(Hg-tnc)gene and FMRFamide-like peptides(Hg-flp)were cloned and construct virus induced gene silencing(VlGS)vectors. The target?genes Hg-flp and Hg-tnc can be obtained from H. glycines by RT-PCR and then ligated to tobacco rattle virus(TRV)?vector pYY13. Then the constructed vectors were transformed into DH5α and identified by PCR and sequencing. Sequence analysis indicates that the sequences of H. glycines Hg-tnc and Hg-tnc were approximately 1 000 bp and 700?bp in length and we successfully constructed recombinant vectors pYY13-Hg-flp and pYY13-Hg-tnc. This work laid?the foundations for soybean resistance breeding and the function reseach on Hg-flp and Hg-tnc.

Key words: Virus induced gene silencing(VlGS), Heterodera glycines Ichinohe, Troponin C, FMRFamide-like peptides, Tobacco rattle virus(TRV)

CLC Number:

S435.651

ZHANG Yi, RUAN Jiu-xiao, MA Xiao-meng, FU Lu-lu, QIAO Yue-e, DUAN Su-juan, MENG Ping, LI Cheng-wei. Identification and Construction of the Recombinant Vector of Virus Induced Gene Silencing of Soybean Heterodera glycines Ichinohe[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 223-226. doi: 10.3969/j.issn.1000-7091.2012.03.043.

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