Abstract: The cross was made between resistant variety Huipizhi Heidou ( ZDD2315 ) and susceptible variety Liaodou 15 to Heterodera glycines race 3. Applying the F 4 separated population from the cross of Huipizhi Heidou and Liaodou 15 as test materials according to Bulked segregant analysis ( BSA ) to study differential proteomic. The total proteins were extracted by TCA/acetone,two-dimensional gel electrophoresis and mass spectrometry were em-ployed to analyze proteins extracted from roots of soybean differentially expressed induced by Heterodera glycines. The results showed that there were 367 and 372 protein spots isolated in silver nitrate stained 2-D gels in resistant and sensitive samples,the 2-D gels were analyzed by ImageMaster 2D Platinum software,there were 6 special pro-tein spots,13 twofold upper protein spots,4 twofold lower protein spots in resistant samples,4 special protein spots in sensitve samples. 16 protein spots were identified by MALDI-TOF-MS and blast in NCBI, and 7 protein spots were from soybean,11 protein spots weren't identified due to the low scores.

Key words: Soybean, Heterodera glycines, Two-dimensional gel electrophoresis, Mass spectrometry

CLC Number: 

• S435.651