Effect of Nitrogen Fertilizer on Soybean Cyst Nematode Disease

doi:10.7668/hbnxb.20192883

Abstract

Abstract:

In order to explore the control effect of nitrogen fertilizer on soybean cyst nematode disease,pot experiment and field experiment were carried out to analyze the effect of nitrogen fertilizer on the egg hatching,nematode development of soybean cyst nematode and the yield of soybean. Four nitrogen application levels were set as 0.016,0.032,0.048,0.064 g/kg soil,respectively,to analyze the effect of soil eluviating solution and root exudate solution on egg hatching. In the field experiment,four nitrogen application levels were set as 22.50,56.25,67.50,78.75 kg/ha,respectively,to analyze the effect of nitrogen fertilizer on the development of nematode. Treatment without nitrogen fertilizer was used as a blank control in all experiments. The results of pot experiment showed that both soil eluviating solution and root exudate solution could significantly improve egg hatching inhibition rate of H. glycines after nitrogen fertilizer application. The soil eluviating solution of 0.032,0.064 g/kg soil had the best inhibition effect on H. glycines egg hatching,reaching at 34.21% and 29.31%,respectively,the root exudate solution of 0.064 g/kg soil had the best inhibition effect on egg hatching of H. glycines,reaching 55.09%. The field experiment showed that proper application of nitrogen fertilizer could significantly decrease cyst number but increase soybean yield. Treatment with 56.25 kg/ha nitrogen fertilizer had the best control effect on nematodes and the highest yield of soybean,with cyst number decreased by 25.29% and the yield of soybean increased by 14.75%. However,the number of nematode in the control increased by 30.77%. Therefore,application of nitrogen fertilizer is an economical and safe control method to improve the yield and quality of soybean.

Key words: Heterodera glycines, Nitrogen fertilizer, Soil eluviating solution, Root exudate, Nematode control

JIA Jianping, LI Jinhong, YU Jingwen, YU Xiyue, PENG Deliang, LI Huixia, HUANG Wenkun. Effect of Nitrogen Fertilizer on Soybean Cyst Nematode Disease[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 198-205. doi: 10.7668/hbnxb.20192883.

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References 57

[1]	韩少杰, 郑经武. 寄主对大豆孢囊线虫抗性相关基因功能研究进展[J]. 生物技术通报, 2021, 37(7):14-24. doi: 10.13560/j.cnki.biotech.bull.1985.2021-0627. doi: 10.13560/j.cnki.biotech.bull.1985.2021-0627
	Han S J, Zheng J W. Research advances on the functional study of host resistance genes to Heterodera glycines[J]. Biotechnology Bulletin, 2021, 37(7):14-24.
[2]	孟凡立, 于瑾瑶, 李春杰, 黄铭慧, 赵磊, 王旋, 姜野, 秦瑞峰, 王从丽. 东北地区大豆孢囊线虫病发生和防控技术研究进展[J]. 东北农业大学学报, 2022, 53(1):87-94. doi: 10.19720/j.cnki.issn.1005-9369.2022.01.010. doi: 10.19720/j.cnki.issn.1005-9369.2022.01.010
	Meng F L, Yu J Y, Li C J, Huang M H, Zhao L, Wang X, Jiang Y, Qin R F, Wang C L. Research progress on occurrence and management of soybean cyst nematode in Northeast China[J]. Journal of Northeast Agricultural University, 2022, 53(1):87-94.
[3]	Rocha L F, Gage K L, Pimentel M F, Bond J P, Fakhoury A M. Weeds hosting the soybean cyst nematode(Heterodera glycines ichinohe):Management implications in agroecological systems[J]. Agronomy, 2021, 11(1):146. doi: 10.3390/agronomy11010146. doi: 10.3390/agronomy11010146 URL
[4]	Legner C M, Tylka G L, Pandey S. Robotic agricultural instrument for automated extraction of nematode cysts and eggs from soil to improve integrated pest management[J]. Scientific Reports, 2021, 11:3212. doi: 10.1038/s41598-021-82261-w. doi: 10.1038/s41598-021-82261-w URL
[5]	陈倩, 张露源, 陈伯昌, 吴海燕. 大豆孢囊线虫生防菌株Myrothecium verrucaria ZW-2发酵条件优化及活性物质分析[J]. 生物技术通报, 2021, 37(7):127-136. doi: 10.13560/j.cnki.biotech.bull.1985.2021-0454. doi: 10.13560/j.cnki.biotech.bull.1985.2021-0454
	Chen Q, Zhang L Y, Chen B C, Wu H Y. Optimization of fermentation conditions of Myrothecium verrucaria ZW-2,a biocontrol strain against Heterodera glycines and analysis of active substances[J]. Biotechnology Bulletin, 2021, 37(7):127-136.
[6]	王宁. 大豆孢囊线虫(Heterodera glycines)溶菌酶基因克隆及功能初步分析[D]. 北京: 中国农业科学院, 2019.
	Wang N. Cloning and functional analysis of lysozyme gene of soybean cyst nematode(Heterodera glycines)[D]. Beijing: Chinese Academy of Agricultural Sciences, 2019.
[7]	张瀛东. 大豆孢囊线虫33E05、34B08和Hg-exp-1基因RNAi功能初步分析与甜菜孢囊线虫分子检测研究[D]. 北京: 中国农业科学院, 2018.
	Zhang Y D. Functional analysis of 33E05,34B08 and Hg-exp-1 gene from the soybean cyst nematode(Heterodera glycines)and research on molecular detection technology of sugarbeet cyst nematode(Heterodera schachtii)[D]. Beijing: Chinese Academy of Agricultural Sciences, 2018.
[8]	Bandara A Y, Weerasooriya D K, Bradley C A, Allen T W, Esker P D. Dissecting the economic impact of soybean diseases in the United States over two decades[J]. PLoS One, 2020, 15(4):e0231141. doi: 10.1371/journal.pone.0231141. doi: 10.1371/journal.pone.0231141 URL
[9]	包冬芳, 董海冉, 王俊, 张婵娟, 赵雪, 韩英鹏. 大豆胞囊线虫病抗性候选基因GmRSCN^-6克隆与表达分析[J]. 中国油料作物学报, 2020, 42(5):826-834. doi: 10.19802/j.issn.1007-9084.2019153. doi: 10.19802/j.issn.1007-9084.2019153
	Bao D F, Dong H R, Wang J, Zhang C J, Zhao X, Han Y P. Cloning and expression analysis of candidate gene GmRSCN-6 for resistance to soybean cyst nematode[J]. Chinese Journal of Oil Crop Sciences, 2020, 42(5):826-834.
[10]	符堯, 李静, 张子安, 周园园. 一株抗大豆胞囊线虫芽孢杆菌的分离鉴定[J]. 黑龙江农业科学, 2022(2): 36-40. doi: 10.11942/j.issn1002-2767.2022.02.0036. doi: 10.11942/j.issn1002-2767.2022.02.0036
	Fu Y, Li J, Zhang Z A, Zhou Y Y. Separation and identification of Bacillus sp. antagonistic to soybean cyst nematode[J]. Heilongjiang Agricultural Sciences, 2022(2):36-40.
[11]	汪瑞. α-SNAP互作蛋白在大豆与大豆孢囊线虫互作中的机制研究[D]. 武汉: 华中农业大学, 2019. doi: 10.27158/d.cnki.ghznu.2019.000951. doi: 10.27158/d.cnki.ghznu.2019.000951
	Wang R. Functional characterization of alpha-SNAP-associated proteins in soybean and soybean cyst nematode interaction[D]. Wuhan: Huazhong Agricultural University, 2019.
[12]	祝群. GmMYB15在大豆与大豆孢囊线虫互作中的机制研究[D]. 武汉: 华中农业大学, 2021.
	Zhu Q. Function characterization of GmMYB15 insoybean and soybean cystnematode interaction[D]. Wuhan: Huazhong Agricultural University, 2021.
[13]	黄铭慧. 大豆对孢囊线虫Heterodera glycines的分子遗传抗性机制研究[D]. 哈尔滨: 中国科学院大学, 2021. doi: 10.27536/d.cnki.gccdy.2021.000010. doi: 10.27536/d.cnki.gccdy.2021.000010
	Huang M H. Research on molecular genetic mechanism of resistance to Heterodera glycines in soybean[D]. Harbin: Chinese Academy of Sciences, 2021.
[14]	罗宁, 刘永刚, 李惠霞, 李焕宇, 陈秀菊. 不同杀线剂对大豆孢囊线虫病的防治效果[J]. 陕西农业科学, 2020, 66(1):10-14. doi: 10.3969/j.issn.0488-5368.2020.01.004. doi: 10.3969/j.issn.0488-5368.2020.01.004
	Luo N, Liu Y G, Li H X, Li H Y, Chen X J. Effect of different nematicides on cereal soybean cystnematode of white[J]. Shaanxi Journal of Agricultural Sciences, 2020, 66(1):10-14.
[15]	姜伟, 张海英, 李金鸿, 连芸芸, 吴锦, 刘永刚, 李惠霞. 5种药剂对大豆孢囊线虫孵化及2龄幼虫室内毒力的影响[J]. 甘肃农业大学学报, 2021, 56(4):36-42. doi: 10.13432/j.cnki.jgsau.2021.04.006. doi: 10.13432/j.cnki.jgsau.2021.04.006
	Jiang W, Zhang H Y, Li J H, Lian Y Y, Wu J, Liu Y G, Li H X. Effects of five agents on hatching of Heterodera glycines and indoor virulence of second-stage juveniles[J]. Journal of Gansu Agricultural University, 2021, 56(4):36-42.
[16]	葛逢勇. 抗大豆孢囊线虫4号生理小种的PI437654突变体的筛选与全基因组测序[D]. 北京: 中国农业科学院, 2018.
	Ge F Y. Screening and whole genome sequencing of the mutants of PI437654 conferring resistance to race 4 of soybean cyst nematode[D]. Beijing: Chinese Academy of Agricultural Sciences, 2018.
[17]	耿晶晶, 杨艺萌, 尹添, 赵增旗, 徐玉梅. 大豆孢囊线虫侵染绿豆的国内首次报道[J]. 山西农业大学学报(自然科学版), 2022, 42(1):90-97. doi: 10.13842/j.cnki.issn1671-8151.202109012. doi: 10.13842/j.cnki.issn1671-8151.202109012
	Geng J J, Yang Y M, Yin T, Zhao Z Q, Xu Y M. First report of Heterodera glycines infecting Vigna radiata in China[J]. Journal of Shanxi Agricultural University (Natural Science Edition), 2022, 42(1):90-97.
[18]	McCoy J M, Kaur G, Golden B R, Orlowski J M, Cook D, Bond J A, Cox M S. Nitrogen fertilization of soybean in Mississippi increases seed yield but not profitability[J]. Agronomy Journal, 2018, 110(4):1505-1512. doi: 10.2134/agronj2017.05.0271. doi: 10.2134/agronj2017.05.0271 URL
[19]	胡田田, 崔晓路, 李梦月, 陆军胜, 罗利华, 陈绍民. 不同氮肥增效剂和水氮用量对冬小麦产量的影响[J]. 农业机械学报, 2021, 52(4):302-310. doi: 10.6041/j.issn.1000-1298.2021.04.032. doi: 10.6041/j.issn.1000-1298.2021.04.032
	Hu T T, Cui X L, Li M Y, Lu J S, Luo L H, Chen S M. Effect of different nitrogen fertilizer synergists,water and nitrogen amount on winter wheat yield[J]. Transactions of the Chinese Society for Agricultural Machinery, 2021, 52(4):302-310.
[20]	德科加, 周青平, 刘文辉, 徐成体, 王德利. 施氮量对青藏高原燕麦产量和品质的影响[J]. 中国草地学报, 2007, 29(5):43-48.
	De K J, Zhou Q P, Liu W H, Xu C T, Wang D L. Effects of nitrogen application on the yield and quality of oat in Qinghai-Tibet plateau[J]. Chinese Journal of Grassland, 2007, 29(5):43-48.
[21]	赵灿, 刘光明, 戴其根, 许轲, 高辉, 霍中洋. 氮肥对水稻产量、品质和氮利用效率的影响研究进展[J]. 中国稻米, 2022, 28(1):48-52,57. doi: 10.3969/j.issn.1006-8082.2022.01.010. doi: 10.3969/j.issn.1006-8082.2022.01.010
	Zhao C, Liu G M, Dai Q G, Xu K, Gao H, Huo Z Y. Research progress on the effects of nitrogen fertilizer on rice yield,quality and nitrogen use efficiency[J]. China Rice, 2022, 28(1):48-52,57.
[22]	朱桂玉, 区惠平, 何佳, 顾明华. 免耕水稻在3414试验方案中氮磷钾配施的肥料效应研究[J]. 中国土壤与肥料, 2011(5):48-52. doi: 10.3969/j.issn.1673-6257.2011.05.009. doi: 10.3969/j.issn.1673-6257.2011.05.009
	Zhu G Y, Ou H P, He J, Gu M H. The research of fertilizer effect on the no-tillage cultivated rice based on experiment of 3414 design[J]. Soil and Fertilizer Sciences in China, 2011(5):48-52.
[23]	胥雅馨, 徐玥, 李玲, 黄兴军, 王德胜, 吴全忠, 陈国栋, 翟云龙. 氮肥与根瘤菌配施对南疆春大豆结瘤和生长的影响[J]. 大豆科学, 2021, 40(2):241-248. doi: 10.11861/j.issn.1000-9841.2021.02. 0241. doi: 10.11861/j.issn.1000-9841.2021.02. 0241
	Xu Y X, Xu Y, Li L, Huang X J, Wang D S, Wu Q Z, Chen G D, Zhai Y L. Effect of nitrogen fertilizer and Rhizobium inoculation on nodulation and growth of southern Xinjiang spring soybean[J]. Soybean Science, 2021, 40(2):241-248.
[24]	刘志强, 王建立, 赵景云, 仇永康. 氮肥用量和种植密度对大豆产量及品质的影响[J]. 农业工程技术, 2021, 41(32):17,21. doi: 10.16815/j.cnki.11-5436/s.2021.32.009. doi: 10.16815/j.cnki.11-5436/s.2021.32.009
	Liu Z Q, Wang J L, Zhao J Y, Qiu Y K. Effects of nitrogen application rate and planting density on yield and quality of soybean[J]. Agricultural Engineering Technology, 2021, 41(32):17,21.
[25]	阮维斌, 王敬国, 张福锁. 根际微生态系统中的大豆胞囊线虫[J]. 植物病理学报, 2002, 32(3):200-205. doi: 10.13926/j.cnki.apps.2002.03.002. doi: 10.13926/j.cnki.apps.2002.03.002
	Ruan W B, Wang J G, Zhang F S. The soybean cyst nematode(Heterodera glycines)in rhizosphere micro-ecologic-system[J]. Acta Phytopathologica Sinica, 2002, 32(3):200-205.
[26]	张福锁, 曹一平. 根际微生态系统养分有效性及植物适应性机理[J]. 土壤, 1993, 25(5):260-262,270.
	Zhang F S, Cao Y P. Nutrient availability of rhizosphere micro-ecosystem and the mechanism of plant adaptability[J]. Soils, 1993, 25(5):260-262,270.
[27]	闫飞, 杨振明, 邹永久. 大豆连作障碍中的生化互作效应[J]. 大豆科学, 1998, 17(2):147-152.
	Yan F, Yang Z M, Zou Y J. Allelopathy on barrier of soybean continuous cropping[J]. Soybean Science, 1998(2):147-152.
[28]	d'Arcy-Lameta A. Study of soybean and lentil root exudates[J]. Plant and Soil, 1986, 92(1):113-123. doi: 10.1007/BF02372272. doi: 10.1007/BF02372272 URL
[29]	Peters N K, Long S R. Alfalfa root exudates and compounds which promote or inhibit induction of Rhizobium meliloti nodulation genes[J]. Plant Physiology, 1988, 88(2):396-400. doi: 10.1104/pp.88.2.396. doi: 10.1104/pp.88.2.396 pmid: 16666315
[30]	Maxwell C A, Phillips D A. Concurrent synthesis and release of nod-gene-inducing flavonoids from alfalfa roots[J]. Plant Physiology, 1990, 93(4):1552-1558. doi: 10.1104/pp.93.4.1552. doi: 10.1104/pp.93.4.1552 pmid: 16667655
[31]	Hartwig U A, Maxwell C A, Joseph C M, Phillips D A. Chrysoeriol and luteolin released from alfalfa seeds induce nod genes in Rhizobium meliloti[J]. Plant Physiology, 1990, 92(1):116-122. doi: 10.1104/pp.92.1.116. doi: 10.1104/pp.92.1.116 pmid: 16667231
[32]	Tefft P M, Bone L W. Plant-induced hatching of eggs of the soybean cyst nematode Heterodera glycines[J]. Journal of Nematology, 1985, 17(3):275-279. doi: 10.1007/BF00987863. doi: 10.1007/BF00987863 pmid: 19294094
[33]	Sikora E J, Noel G R. Hatch and emergence of Heterodera glycines in root leachate from resistant and susceptible soybean cultivars[J]. Journal of Nematology, 1996, 28(4):501-509. doi: 10.2307/1565709. doi: 10.2307/1565709 pmid: 19277168
[34]	Schmitt D P, Riggs R D. Influence of selected plant species on hatching of eggs and development of juveniles of Heterodera glycines[J]. Journal of Nematology, 1991, 23(1):1-6. doi: 10.1016/0022-2011(91)90056-V. doi: 10.1016/0022-2011(91)90056-V pmid: 19283087
[35]	许艳丽. 土壤环境对大豆胞囊线虫卵孵化影响及线虫分子诊断研究[D]. 哈尔滨: 东北农业大学, 2004. doi: 10.7666/d.y631969. doi: 10.7666/d.y631969
	Xu Y L. The effects of soil environment on soybean cyst nematode Heterodera glycinese egg hatch and nematode molecular diagnosis[D]. Harbin: Northest Agricultural University, 2004.
[36]	许艳丽, 陈伊里, 司兆胜, 李兆林, 李春杰, 温广月. 不同茬口条件下的作物根渗出物对大豆胞囊线虫(Heterodera glycines)卵孵化影响[J]. 植物病理学报, 2004, 34(6):481-486. doi: 10.13926/j.cnki.apps.2004.06.001. doi: 10.13926/j.cnki.apps.2004.06.001
	Xu Y L, Chen Y L, Si Z S, Li Z L, Li C J, Wen G Y. The effects of the root diffusate of different crops from different rotation systems on the egg hatch of soybean cyst nematode Heterodera glycines[J]. Acta Phytopathologica Sinica, 2004, 34(6):481-486.
[37]	李惠霞, 刘永刚, 朱锐东, 柳永娥, 彭德良. 甘肃省小麦禾谷孢囊线虫的发生及分布[J]. 植物保护, 2016, 42(3):170-174. doi: 10.3969/j.issn.0529-1542.2016.03.030. doi: 10.3969/j.issn.0529-1542.2016.03.030
	Li H X, Liu Y G, Zhu R D, Liu Y E, Peng D L. Occurrence and distribution of Heterodera avenae in Gansu Province[J]. Plant Protection, 2016, 42(3):170-174.
[38]	王雪, 段玉玺, 陈立杰, 王媛媛. 大豆根系分泌物中氨基酸组分与抗大豆胞囊线虫的相关性研究[J]. 沈阳农业大学学报, 2008, 39(6):677-681. doi: 10.3969/j.issn.1000-1700.2008.06.009. doi: 10.3969/j.issn.1000-1700.2008.06.009
	Wang X, Duan Y X, Chen L J, Wang Y Y. Correlation analysis of amino acids components in soybean cultivars root exudates and resistance to soybean cyst nematode[J]. Journal of Shenyang Agricultural University, 2008, 39(6):677-681.
[39]	Tenuta M, Lazarovits-G Ammonia and nitrous acid from nitrogenous amendments kill the microsclerotia of Verticillium dahliae[J]. Phytopathology, 2002, 92(3):255-264. doi: 10.1094/PHYTO.2002.92.3.255. doi: 10.1094/PHYTO.2002.92.3.255 pmid: 18943996
[40]	文才艺, 吴元华, 张艳红. 烟草感染马铃薯Y病毒脉坏死株系后PPO活性及其同工酶变化的研究[J]. 华中农业大学学报, 2000, 19(4):328-330. doi: 10.3321/j.issn:1000-2421.2000.04.006. doi: 10.3321/j.issn:1000-2421.2000.04.006
	Wen C Y, Wu Y H, Zhang Y H. Changes of polyphenoloxidease activity and isozyme in tobacco infected with Potato virus Y-vein necrosis strain[J]. Journal of Huazhong Agricultural University, 2000, 19(4):328-330.
[41]	金霞, 赵正雄, 李忠环, 陈荣平, 徐天养, 解玉萍, 许龙, 欧阳进, 徐发华. 不同施氮量烤烟赤星病发生与发病初期氮营养、生理状况关系研究[J]. 植物营养与肥料学报, 2008, 14(5):940-946. doi: 10.3321/j.issn:1008-505X.2008.05.019. doi: 10.3321/j.issn:1008-505X.2008.05.019
	Jin X, Zhao Z X, Li Z H, Chen R P, Xu T Y, Xie Y P, Xu L, Ouyang J, Xu F H. Study on the relationship between the content of nitrogen and some biophysiological substances at topping and the occurrence of brown spot of tobacco applied with different amount of nitrogen[J]. Journal of Plant Nutrition and Fertilizers, 2008, 14(5):940-946.
[42]	李海燕, 刘惕若, 甄艳. 辣椒品种对疫病的抗性研究--氨酸、丙二醛与可溶性糖在抗病中的作用[J]. 中国农学通报, 2006, 22(11):315-317. doi: 10.3969/j.issn.1000-6850.2006.11.075. doi: 10.3969/j.issn.1000-6850.2006.11.075
	Li H Y, Liu T R, Zhen Y. Study on the resistance to Phytophthora blight of pepper and the effect of pro,MAD and dissolubility sugar[J]. Chinese Agricultural Science Bulletin, 2006, 22(11):315-317.
[43]	徐宏, 崔晓东, 倪闪闪, 罗家俊, 喻婧, 张莉. 不同施肥模式对小麦产量和效益的影响[J]. 农业科技通讯, 2021(9):144-146. doi: 10.3969/j.issn.1000-6400.2021.09.048. doi: 10.3969/j.issn.1000-6400.2021.09.048
	Xu H, Cui X D, Ni S S, Luo J J, Yu J, Zhang L. Effect of different fertilization modes on wheat yield and benefit[J]. Bulletin of Agricultural Science and Technology, 2021(9):144-146.
[44]	Xiang W H, Zhao L, Xu X, Qin Y H, Yu G H. Mutual information flow between beneficial microorganisms and the roots of host plants determined the bio-functions of biofertilizers[J]. American Journal of Plant Sciences, 2012, 3(8):1115-1120. doi: 10.4236/ajps.2012.38134. doi: 10.4236/ajps.2012.38134 URL
[45]	山楠. 京郊小麦-玉米轮作体系氮素利用与损失研究[D]. 保定: 河北农业大学, 2014.
	Shan N. Nitrogen utilization and loss in winter wheat-summer maize rotation system of Beijing suburb[D]. Baoding: Hebei Agricultural University, 2014.
[46]	史校艳, 王志强, 谷庆昊, 常明娟, 胡俊杰, 任永哲, 辛泽毓, 林同保. 施氮量对不同茬口冬小麦产量及氮肥利用率的影响[J]. 生态学杂志, 2019, 38(7):2041-2048. doi: 10.13292/j.1000-4890.201907.035. doi: 10.13292/j.1000-4890.201907.035
	Shi X Y, Wang Z Q, Gu Q H, Chang M J, Hu J J, Ren Y Z, Xin Z Y, Lin T B. Effects of nitrogen application on yield and nitrogen use efficiency of winter wheat with different former crops[J]. Chinese Journal of Ecology, 2019, 38(7):2041-2048.
[47]	杨卫星, 袁虹霞, 孙炳剑, 邢小萍, 张飞跃, 殷宪平, 王振跃, 李洪连. 不同施肥种类及施用量对小麦禾谷胞囊线虫病发生的影响[J]. 植物病理学报, 2008, 38(6):613-618. doi: 10.13926/j.cnki.apps.2008.06.005. doi: 10.13926/j.cnki.apps.2008.06.005
	Yang W X, Yuan H X, Sun B J, Xing X P, Zhang F Y, Yin X P, Wang Z Y, Li H L. Effects of fertilization on cereal cyst nematode of wheat[J]. Acta Phytopathologica Sinica, 2008, 38(6):613-618.
[48]	黄菁华. 水稻品种和施肥影响下褐飞虱和根系植食线虫的相互作用机制研究[D]. 南京: 南京农业大学, 2014.
	Huang J H. Interactions between brown planthopper and root-feeding nematodes affected by cultivar and fertilizer management[D]. Nanjing: Nanjing Agricultural University, 2014.
[49]	常海娜. 连作年限和施肥措施对番茄生长及根结线虫危害的影响[D]. 南京: 南京农业大学, 2019. doi: 10.27244/d.cnki.gnjnu.2019.000347. doi: 10.27244/d.cnki.gnjnu.2019.000347
	Chang H N. Effects of monocropping duration and fertilization regimes on tomato growth and root-knot nematodes[D]. Nanjing: Nanjing Agricultural University, 2019.
[50]	张平珍, 张克厚, 陈莺, 陈靖萍, 罗健科, 王泽宇. 灌溉条件下燕麦氮、磷、钾配方施肥效应分析及与产量回归模型的建立[J]. 作物杂志, 2021(5):101-107. doi: 10.16035/j.issn.1001-7283.2021.05.015. doi: 10.16035/j.issn.1001-7283.2021.05.015
	Zhang P Z, Zhang K H, Chen Y, Chen J P, Luo J K, Wang Z Y. The effects analysis of nitrogen,phosphorus and potassium fertilization on oat and establishment of yield regression model under irrigation condition[J]. Crops, 2021(5):101-107.
[51]	Hoffland E, Jeger M J, van Beusichem M L. Effect of nitrogen supply rate on disease resistance in tomato depends on the pathogen[J]. Plant and Soil, 2000, 218(1/2):239-247. doi: 10.1023/A:1014960507981. doi: 10.1023/A:1014960507981
[52]	Pozio E, Armignacco O, Ferri F, Angeles M, Morales G. Influence analysis of application of NPK fertilizer on epidemics of rapeseed Sclerotinia stem rot[J]. Chinese Journal of Oil Crop Sciences, 2013, 126(1):54-62. doi: 10.1016/j.actatropica.2013.01.005. doi: 10.1016/j.actatropica.2013.01.005
[53]	刘维志. 植物病原线虫学[M]. 北京: 中国农业出版社, 2000:166-167.
	Liu W Z. Plant pathogenic nematology[M]. Beijing: China Agriculture Press, 2000:166-167.
[54]	Dakwa J T, Murray D B, Dakwa J T. The effects of shade and NPK fertilizers on the incidence of cocoa black pod disease in Ghana[J]. Ghana Journal of Agricultural Science, 1976, 9(3):179-184.
[55]	迟超. 大豆高效施肥技术研究进展[J]. 现代化农业, 2013(10):9-12. doi: 10.3969/j.issn.1001-0254.2013.10.006. doi: 10.3969/j.issn.1001-0254.2013.10.006
	Chi C. Research progress of soybean high-efficiency fertilization technology[J]. Modernizing Agriculture, 2013(10):9-12.
[56]	Kirwa H K, Murungi L K, Beck J J, Torto B. Elicitation of differential responses in the root-knot nematode Meloidogyne incognita to tomato root exudate cytokinin,flavonoids,and alkaloids[J]. Journal of Agricultural and Food Chemistry, 2018, 66(43):11291-11300. doi: 10.1021/acs.jafc.8b05101. doi: 10.1021/acs.jafc.8b05101 URL
[57]	Dyer S, Weir R, Cox D, Cheseto X, Torto B, Dalzell J J. Ethylene response factor(ERF)genes modulate plant root exudate composition and the attraction of plant parasitic nematodes[J]. International Journal for Parasitology, 2019, 49(13/14):999-1003. doi: 10.1016/j.ijpara.2019.09.001. doi: 10.1016/j.ijpara.2019.09.001 URL

编号 Number	病株率/% Diseased plant rate	根长/cm Root length	株高/cm Plant height	荚粒数/粒 Seeds number per pod	千粒质量/g 1000-grain weight	理论产量/ (kg/hm²) Yield	增产率/% Yield increase rate
N0.50	22.50±5.87ab	20.05±1.24a	67.30±1.97a	2.27±0.20a	187.20±5.94bc	2 491.50	6.43
N1.25	20.79±4.66b	21.55±0.74a	67.65±2.66a	2.20±0.19a	201.83±3.50a	2 686.20	14.75
N1.50	17.80±3.81b	21.25±1.23a	70.05±2.33a	2.17±0.16a	198.90±2.97a	2 573.10	9.92
N1.75	23.94±4.32ab	20.30±0.87a	65.50±1.67a	2.24±0.30a	193.15±0.99ab	2 555.55	9.17
CK	32.75±6.13a	19.40±0.96a	49.50±1.43b	2.18±0.13a	182.75±1.06c	2 340.90

编号 Number	病株率/% Diseased plant rate	根长/cm Root length	株高/cm Plant height	荚粒数/粒 Seeds number per pod	千粒质量/g 1000-grain weight	理论产量/ (kg/hm²) Yield	增产率/% Yield increase rate
N0.50	22.50±5.87ab	20.05±1.24a	67.30±1.97a	2.27±0.20a	187.20±5.94bc	2 491.50	6.43
N1.25	20.79±4.66b	21.55±0.74a	67.65±2.66a	2.20±0.19a	201.83±3.50a	2 686.20	14.75
N1.50	17.80±3.81b	21.25±1.23a	70.05±2.33a	2.17±0.16a	198.90±2.97a	2 573.10	9.92
N1.75	23.94±4.32ab	20.30±0.87a	65.50±1.67a	2.24±0.30a	193.15±0.99ab	2 555.55	9.17
CK	32.75±6.13a	19.40±0.96a	49.50±1.43b	2.18±0.13a	182.75±1.06c	2 340.90

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