Abstract: This study was performed to identify one isolate with wizened,mottle and vein clearing mosaic symptom collected from greenhouse mung bean(Vigna radiata (L.) Wilczek)in Jangsu.We used electron micrograph,PCR,determination of nucleotide sequence and ELISA.We can see the characteristics of the virus under transmission electron microscopy.We used Potyvirus universal primers for RT-PCR,found the symptom was caused by Bean common mosaic virus(BCMV).This isolate was called BCWV-JAAS.The nucleotides sequences of the genes from the isolate shared homologies from 95% with reported BCMV-HB.Total RNA was amplified by RT-PCR with the specific primers designed on the basis of the reported coat protein gene of Bean common mosaic virus(BCMV).An 1 082 bp gene fragment was amplified from total RNA of isolate,and subsequent sequence analysis indicated that the 862 bp fragment contained complete cp gene which encoded 228 amino acids.By amplification cp genes and construct phylogenetic trees get BCMV-JAAS with other Chinese isolates of BCMV have a close genetic relationship.We performed the virus samples by ELISA,the results shown positive we ensured that was infecting by Bean common mosaic virus(BCMV) to cause the mung bean plant disease.This is the first time found the mung bean was infected by BCMV in China.

Key words: Mung bean, Bean common mosaic virus, Pathogen identify

CLC Number: 

• S435.651