Accessorial Methods to Achieve Full Length cDNA Sequence in Maize Genome and Their Effectiveness Evaluation

doi:10.7668/hbnxb.2008.S1.005

Abstract

Abstract: Take maize DNA Topoisomerase N gene Top1 cloning as an example, three accessorial methods developed for achieving full length cDNA sequence in maize genome, that were step by step approach, conserved domain strategy and BAC technique, were described. The three accessorial methods were also assessed primarily in this study.

Key words: Maize, Full length cDNA, Accessorial method

CLC Number:

S513

WANG Yi-jun, DENG De-xiang, BIAN Yun-long. Accessorial Methods to Achieve Full Length cDNA Sequence in Maize Genome and Their Effectiveness Evaluation[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S1): 19-22. doi: 10.7668/hbnxb.2008.S1.005.

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