Identification of Bacillus sp. Pb-4 and Its Unitilization for Antimicrobial Activity

doi:10.7668/hbnxb.2016.02.036

Abstract

Abstract: A bacillus strain, named as Pb-4, which showed significant antagonistic activity against to many plant pathogenic fungi.The study was in order to determine the taxonomic status and antimicrobial mechanism of Pb-4.The strain was identified as Bacillus amyloliquefaciens based on its morphological and physiological properties and its 16S rRNA sequence.The antibacterial activity was characterized by plate two-way cultivation.Antibacterial compound from the identified strain was separated and purified by ammonium sulfate precipitation, diethyl ether and benzene abstract.Bacillus amyloliquefaciens was isolated that not only has antibacterial activity against F.oxysporum tomato, F.oxysporum cucumber, F.oxysporum watermelon and F.oxysporum green pepper;but also against Phytophthora capsici Leonian and Valsa ceratosperma.The bacteria liquid of the Pb-4 strain showed that strong antifungal activity against spore germination and hyphal growth of tomato wilt pathogen.The bacteria fluid to fusarium wilt diseases of tomato inhibition of growth rate was 93%. The antimicrobial activity of 100, 121℃ high temperature treatment of untreated bacteria liquid of 98.9% and 80.1%.From the bacteria liquid extract of proteins, enzymes, and antibiotic active substances, had strong growth inhibition effect for Fusarium oxysporum.The results can provide a strong theoretical basis for the wide application of Pb-4 in the prevention and control of agricultural pathogenic bacteria.

Key words: Bacillus amyloliquefaciens, Fusarium wilt, Antagonistic bacteria, Antimicrobial activity

CLC Number:

S432

GUO Jun, WU Ailian, YAN Min, LI Wei, CHI Xiurong, DING Yuchuan, JIAO Xiaoyan. Identification of Bacillus sp. Pb-4 and Its Unitilization for Antimicrobial Activity[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 224-230. doi: 10.7668/hbnxb.2016.02.036.

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