Sequence Analysis of the Hemagglutinin Gene of Canine distemper virus from Vaccinated Dogs,Minks and Foxes

doi:10.7668/hbnxb.2016.02.037

Abstract

Abstract: In order to understand whether new genotype emerged, we detected Canine distemper virus(CDV) in immunized dogs, breeding foxes and minks in different regions by amplification of nucleocapsid(N) and hemagglutinin(H) gene.13 strains from CDV-positive cases(8 minks, 2 foxes and 3 dogs) were sequenced.The results showed that the 13 Chinese strains shared 99.1%-100.0% nucleotide identity.Compared with those of other Chinese CDV strains isolated before 2012, four consistent amino acid changes, P200S, M263I, I542N and Y549H, were observed in the H coding sequence of CDV isolated from vaccinated animals, which contribute to an additional N-glycosylation site and antigenic epitope.All of the strains obtained from 13 samples were characterized as genotype Asia-1 according to the sequence analysis of the complete H gene, which was the sole genotype in minks in China.These findings provide a comprehensive understanding of current immune failure and the molecular epidemiology of CDV in China.

Key words: Canine distemper virus, Hemagglutinin gene(H), Vaccination failure

CLC Number:

S85
S432.4+1

HUANG Juan, SUI Xiaomei, WANG Yinan, SHAN Hu. Sequence Analysis of the Hemagglutinin Gene of Canine distemper virus from Vaccinated Dogs,Minks and Foxes[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 231-238. doi: 10.7668/hbnxb.2016.02.037.

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References

[1] Appel M J,Yates R A,Foley G L,et al.Canine distemper epizootic in lions,tigers,and leopards in North America[J].Journal of Veterinary Diagnostic Investigation,1994,6(3):277-288.
[2] Sakai K,Yoshikawa T,Seki F,et al.Canine distemper virus associated with a lethal outbreak in monkeys can readily adapt to use human receptors[J].Journal of Virology,2013,87(12):7170-7175.
[3] Seimon T A,Miquelle D G,Chang T Y,et al.Canine distemper virus:an emerging disease in wild endangered Amur tigers(Panthera tigris altaica)[J].mBio,2013,4(4).pii:e00410-13.
[4] Si W,Zhou S,Wang Z,et al.A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus[J].Virology Journal,2010,7:86.
[5] Zhao J J,Zhang H L,Bai X E,et al.Emergence of canine distemper virus strains with two amino acid substitutions in the haemagglutinin protein,detected from vaccinated carnivores in North-Eastern China in 2012-2013[J].Veterinary Journal,2014,200(1):191-194.
[6] Rima B K.The proteins of morbilliviruses[J].Journal of General Virology,1983,64(6):1205-1219.
[7] Bolt G,Jensen T D,Gottschalck E,et al.Genetic diversity of the attachment(H)protein gene of current field isolates of Canine distemper virus[J].Journal of General Virology,1997,78(2):367-372.
[8] Martella V,Cirone F,Elia G,et al.Heterogeneity within the hemagglutinin genes of Canine distemper virus(CDV)strains detected in Italy[J].Veterinary Microbiology,2006,116(4):301-309.
[9] Espinal M A,Díaz F J,Ruiz-Saenz J.Phylogenetic evidence of a new Canine distemper virus lineage among domestic dogs in Colombia,South America[J].Veterinary Microbiology,2014,172(1-2):168-176.
[10] Guo L,Yang S L,Wang C D,et al.Phylogenetic analysis of the haemagglutinin gene of Canine distemper virus strains detected from giant panda and raccoon dogs in China[J].Virology Journal,2013,10:109.
[11] Bi Z W,Wang Y S,Wang X L,et al.Phylogenetic analysis of Canine distemper virus in domestic dogs in Nanjing,China[J].Archives of Virology,2015,160(2):523-527.
[12] Zhao J J,Yan X J,Chai X L,et al.Phylogenetic analysis of the haemagglutinin gene of Canine distemper virus strains detected from breeding foxes,raccoon dogs and minks in China[J].Veterinary Microbiology,2010,140(1/2):34-42.
[13] Li W,Li T,Liu Y,et al.Genetic characterization of an isolate of Canine distemper virus from a Tibetan Mastiff in China[J].Virus Genes,2014.49(1):45-57.
[14] Tamura K,Peterson D,Peterson N,et al.MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Molecular Biology and Evolution,2011,28(10):2731-2739.
[15] Budaszewski R F,Pinto L D,Weber M N,et al.Genotyping of Canine distemper virus strains circulating in Brazil from 2008 to 2012[J].Virus Research,2014,180:76-83.
[16] Mochizuki M,Hashimoto M,Hagiwara S,et al.Genotypes of Canine distemper virus determined by analysis of the hemagglutinin genes of recent isolates from dogs in Japan[J].Journal of Clinical Microbiology,1999,37(9):2936-2942.
[17] Ohishi K,Suzuki R,Maeda T,et al.Recent host range expansion of Canine distemper virus and variation in its receptor,the signaling lymphocyte activation molecule,in carnivores[J].Journal of Wildlife Diseases,2014,50(3):596-606.
[18] Han G Z,Liu X P,Li S S.Cross-species recombination in the haemagglutinin gene of Canine distemper virus[J].Virus Research,2008,136(1/2):198-201.
[19] Cottrell W O,Keel M K,Brooks J W,et al.First report of clinical disease associated with Canine distemper virus infection in a wild black bear(ursus americana)[J].Journal of Wildlife Diseases,2013,49(4):1024-1027.
[20] Jameson B A,Wolf H.The antigenic index:a novel algorithm for predicting antigenic determinants[J].Computer Applications in the Biosciences,1988,4(1):181-186.
[21] Sawatsky B,von Messling V.Canine distemper viruses expressing a hemagglutinin without N-glycans lose virulence but retain immunosuppression[J].Journal of Virology,2010,84(6):2753-2761.
[22] Ohashi K,Iwatsuki K,Nakamura K,et al.Molecular identification of a recent type of canine distempervirus in Japan by restriction fragment length polymorphism[J].Journal of Veterinary Medical Science,1998,60(11):1209-1212.
[23] Iwatsuki K,Tokiyoshi S,Hirayama N,et al.Antigenic differences in the H proteins of Canine distemper viruses[J].Veterinary Microbiology,2000,71(3/4):281-286.
[24] Nikolin V M,Wibbelt G,Michler F,et al.Susceptibility of carnivore hosts to strains of Canine distemper virus from distinct genetic lineages[J].Veterinary Microbiology,2012,156(1/2):45-53.

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