The Localization of Interleukin 2 in Chinese Yellow Quail Spleen

doi:10.7668/hbnxb.201751525

Abstract

Abstract: In order to explore the mechanism of IL-2 immunoregulation in the spleen of Chinese yellow feather quail,observe the specific distribution characteristics of IL-2 in the spleen and the possible changes of IL-2 in the spleen with the age,so as to provide a theoretical basis for the future study of how IL-2 plays a role in the immune response of quail.Ten healthy yellow feathered quails of 0,1,2,3,4,5 and 6 weeks old were randomly selected.Then the spleen of quails was taken and the IL-2 was histologically located by immunohistochemistry.The morphology and distribution of the positive cells in the spleen of different weeks old were observed.The data were analyzed by data analysis software to find out whether the IL-2 changed with the increase of weeks old.The results showed that the positive cells mainly appeared near the peri-arterial lymphoid sheath,some gathered in the peri-ellipsoidal lymphatic sheath,and the others were scattered in the splenic cord,and the expression of IL-2 increased with the age of 6 weeks,and the change of the number of positive cells was clear,showing an upward trend as a whole,with regularity.Tfh cells and B lymphocytes played different roles in the immune function of spleen.IL-2 might be expressed in Tfh cells migrated to PELS or in B lymphocytes near PELS,indicating that the distribution of IL-2 positive cells was closely related to the effect of IL-2 on lymphocytes.The structure and function of spleen developed with age,and the number and immune intensity of IL-2 positive cells increased with age,which indicated that the immune intensity of IL-2 positive cells was related to the perfection of spleen tissue structure and function;These results suggested that IL-2 might be involved in the proliferation and differentiation of spleen T cells and B cells and played an important role in the improvement of spleen immune function.

Key words: Chinese yellow quail, Interleukin 2, Spleen, Localization

CLC Number:

S852.16

HE Yaoyao, HE Min, ZHAO Piao, HE Lingyu, XU Dingxin. The Localization of Interleukin 2 in Chinese Yellow Quail Spleen[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 409-415. doi: 10.7668/hbnxb.201751525.

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