Cross Neutralization of Fiber Protein Against Four Kinds of Serotype fowl adenovirus Prevalent in China

doi:10.7668/hbnxb.20191066

Abstract

Abstract: To certain the effect of Fiber on the cross-neutralization among FAdV-4,FAdV-8a,FAdV-8b,FAdV-11,antisera against Fiber proteins of four Serotype fowl adenovirus were prepared to study the protective effect on the infection of fowl adenovirus with different serotypes at the LMH cell in the study. The recombinant plasmids of pCold-FAdV-Fiber were constructed after fiber gene was amplified by PCR,and the plasmids were transfected into BL21(DE3) and Fiber proteins were expressed. The products expressed were purified after identified by SDS-PAGE and Western Blot. SPF chickens were immunized with purified Fiber proteins and antisera were collected. The neutraliztion of antisera against Fiber proteins and different fowl adenovirus infection were studied on the LMH cells.The results showed that the size of FAdV-4,FAdV-8a,FAdV-8b,FAdV-11 PCR fragment were 1 440,1 575,1 566,1 719 bp differently,and the molecular weight of the proteins expressed were 102,107,107,112 ku. Antisera against Fibers of FAdV-4,FAdV-8b and FAdV-11 only neutralized infection of the virus with same serotype,and antiserum against Fibers of serotype FAdV-8a could not protect the LMH cells from infection of the virus with these four serotype. Comprehensively,Fiber proteins were not directly related to the cross-protection between the four serotypes FAdV viruses.

Key words: FAdV, Fiber protein, Prokaryotic expression, Cross neutralization

CLC Number:

S855.3

LUAN Qingdong, SUN Ju, LIU Junhua, ZHANG Benqing, YIN Yanbo, WANG Jianlin. Cross Neutralization of Fiber Protein Against Four Kinds of Serotype fowl adenovirus Prevalent in China[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(5): 214-219. doi: 10.7668/hbnxb.20191066.

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