Calcium/Calmodulin-dependent Protein Kinase Ⅱ Contributes to HSPTO Expression in Mouse Embryonic Fibroblasts

doi:10.3969/j.issn.1000-7091.2012.04.041

Abstract

Abstract: The objectives of the study were to verify if the CaMK Ⅱ （Ca^2＋/calmodulin-dependent protein kinase Ⅱ ） influences the expression of HSP70 gene in the mouse embryonic fibroblasts （MEF） with heat shock and to clarify its mechanism. The fibroblasts were heat-treated at 37℃,39℃ and 41℃ individually before the expressions of both CaMK Ⅱ and HSF1 were detected by RT-PCR at 0.5,1,1.5 and 2 h of heat shock, respectivelly. Moreover, the fibroblasts were divided into control group and myr-AIP group randomly, and cultured at 37 ℃ and 39℃,respec- tively. The expressions of both HSP70 and HSF1 were detected. The expressions of HSF1 and CaMK Ⅱ mRNA in the MEF treated at 39℃ for 1 h were both extremely significantly higher （P 〈0. 01 ） than that of the control group. There was a significantly （P 〈 0.05） decrease of HSP70 expression in the MEF cultured at 39℃ treated with myr- AlP compared with its blank control. However,there was no difference in HSP70 exoression between the MEF cul- tured at 37℃ with or without myr-AIP. The expressions of HSF1 in the MEF treated with myr-AIP were not signifi- cantly influenced. However,the p-HSF1 expression in the MEF treated with myr-AIP was significantly （P 〈 0. 05） decreased. Moderate heat shock increases both expressions of CaMK Ⅱ and HSF1 in mouse embryonic fibroblasts. CaMK Ⅱ participates in both heat shock response and HSP70 gene expression by p-HSF1.

Key words: Heat shock response, HSP70 CaMK Ⅱ HSF1, Mouse embryonic fibroblasts

CLC Number:

SUN Qi, CONG Xia, SUO Jia-jia, CAO Rong-feng, JIANG Zhong-ling, CUI Kai, GAO Shan-song, TIAN Wen-ru. Calcium/Calmodulin-dependent Protein Kinase Ⅱ Contributes to HSPTO Expression in Mouse Embryonic Fibroblasts[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 213-217. doi: 10.3969/j.issn.1000-7091.2012.04.041.

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