Identification of cry Gene from a Novel Bacillus thuringiensis Isolate SHJ-5

doi:10.7668/hbnxb.2011.04.034

Abstract

Abstract: The novel Bacillus thuringiensis SHJ-5 was isolated from the soil in Heilongjiang province.This paper was a study on the strain SHJ-5,including that the shape of the crystal,the cry-type,protein expression and insecticidal activity.It produced blunt diamond-shaped crystals.It was identified that strain SHJ-5 contained cry1Aa，cry1Ea，cry1Be，cry1Ah，cry1Ai，cry2Ab，cry1I and vip3A gene by PCR-RFLP.SDS-PAGE analysis indicated that the strain produced a 130-150 kDa protein and a 60 kDa protein.Bioassay showed that strain SHJ-5 had high insecticidal activity to Helicoverpa armigera and Plutella xylostella.The corrected mortality to the two target insects was 100% and 96.7%,respectively.

Key words: Bacillus thuringiensis, PCR-RFLP, Clone, Insecticidal activity

CLC Number:

S476 +.12

DU Li-xin, DONG Ming, WANG Rong-yan, MA Ming-ze, WANG Jin-yao, CAO Wei-ping, SONG Jian, FENG Shu-liang. Identification of cry Gene from a Novel Bacillus thuringiensis Isolate SHJ-5[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 194-197. doi: 10.7668/hbnxb.2011.04.034.

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http://www.hbnxb.net/EN/Y2011/V26/I4/194

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