Abstract: Based on the exon 1 and exon 3 of the pig's RBP4 gene, a pair of primers were designed and synthesized, successfully amplified the gene's intron 1 and intron 2, and the identity of RBP4 gene sequences was 80% between pig and human, 78% between pig and mouse, the results showed that the amplification was correct. Polynerous of RBP4 gene in five strains was detected by PCR-RFLP. The strains were involved Yorkshire, Duroc, Landrace, Min pig and Crossbred pig. After genotyping with MspⅠ: there were AA, BB and AB; Except the Yorkshire and Min pig, the Landrace and Crossbred pig, the distribution of other pigs' genotype frequencies were significance (P<0.01). This MspⅠ site in the gene in Landrace, Yorkshire and Crossbred pig population were disequilibrium, in Duroc and Min pig populations were equilibrium. Used the polymerous information content (PIC) to calculate, the five pig breeds appeared normal polymerous (0.5>PIC>0.25). The allele B of the Min pig is advantageous. It is presumed that the allele B is associate with pig's high production character.

Key words: Pig, RBP4 gene, Clone, PCR-RFLP

CLC Number: 

• S828

• Q789