Abstract: A pair of primers was designed according to the sequence of Oleosin protein promoter, which showed seeds oil-body specificity, and a fragment of Oleosin gene in the promoter region was amplified by polymerase chain reaction (PCR) using the genomic DNA of Brassica napus as template. The fragment was cloned into pGMT vector, and a new recombined vector named pGM-T-SOP was obtained after analyzed with PCR and restriction digestion. The results of sequence analysis indicated that this fragment had 95.6% homology compared with the reported promoter in Genbank. A new plant expression vector named pBI121-SOP in which the reporter gene GUS is drived by SOP was constructed after cutting two vectors pGMT-SOP and pBI121 with two restriction enzymes Hind Ⅲ and BamH I, subsequently recovering the small SOP fragment from pGMT-SOP recombined vector and the long fragment excised CaMV35S promoter from pBI121 plant expression vector, and then ligation, transformation and identification. And then foundation has been set up for further research work in expression and function of this promoter.

Key words: Seeds oil-body-specific promoter, Cloning, Brassica napus.

CLC Number: 

• Q789