Construction of Expression Vector for Anti Stripe Virus of Rice by RNA Interference

doi:10.7668/hbnxb.2008.04.003

Abstract

Abstract: By using the sequence of stripe virus published on line and the software of siRNA Target Finder, we ob - tained 168 siRNA segments.After BLAST Scan of rice genome, 6 segments were selected and 2 were chemically synthe - sized and used to construct of expression vector ( pASV1301 -1, pASV1301 -2) intergratedwith pCAMBIA1301 plasmid.The recombined plasmid was successfully transformed into agrobacterium of EHA105,which lay a foundation for the transfor - mation of rice and the prevention of stripe virus disease of rice by RNA interference.

Key words: Rice, RNA interference, Construction of expression vector, Ant- i virus

CLC Number:

S432.4+1

ZHANG Gong, LIU Li-feng, ZHOU Wei, WANG Hai-guang, MA Zhi-ying. Construction of Expression Vector for Anti Stripe Virus of Rice by RNA Interference[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 10-13. doi: 10.7668/hbnxb.2008.04.003.

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References

[1] Fire A,Xu S,Montgomery M K,et al.Polent and specific ge netlc interference by double-stranded RNA in Caenorhabditis elegam[J].Nature,1998,391:806-811.
[2] 朱龙付,张献龙.RANi及其在植物遗传改良中的应用[J].华中农业大学学报,2004,23(4):472-477.
[3] 燕飞,成卓敏.RNA干扰与植物抗病毒[J].生物技术通报,2005(4):1-4.
[4] 孙黛珍,江玲,张迎信,等.8个水稻品种的条纹叶枯病抗性特征[J].中国水稻科学,2006,20(2):219-222.
[5] 曹明霞,卫志明,黄健秋.根癌农杆菌介导的水稻遗传转化[J].植物生理学通讯,2002,38(5):423-427.
[6] 王关林.植物基因工程原理与技术[M].北京:科学出版社,1998.
[7] 邹麟,叶荻庆,张鹏辉.RNAi-DNA表达载体的构建与应用[J].重庆医学,2005,34(9):1392-1394.
[8] 汪承刚,张菊红,谢妤,等.甘蓝型油菜依赖焦磷酸的磷酸果糖激酶(PFK)基因片段克隆及hpRNAi载体构建[J].分子植物育种,2006,4(2):199-204.
[9] 李小平,邓楠,马嫒嫒,等.大豆类受体蛋白激酶基因(rlpk2)RNAi双元表达载体的构建及其转基因[J].分子细胞生物学报,2006,39(1):1-8.
[10] 彭吴,翟英,张芊,等.水稻高效RNA干涉体系的建立及其功能分析[J].中国农业科学,2006,39(9):1729-1735.
[11] Lu Shanfa,SlIi Rui,Cheng-Chung Tsao,et al.RNA silencing in plants by the expression of siRNA duplexes[J].Nucleic Acids Rehash,2004,32(21):2-7.
[12] 张恭,刘立峰,马峙英.RNA干扰技术及其植物抗病毒应用[J].中国农学通报,2007,23(1):42-45.
[13] Wang M B,Abbott D C,waterhouse P M.A sjngle copy of rims derived transgene encoding hairpin RNA give immunity to barley yellow dwarf virus[J].Molecular Plant Pathology,2000,1:347-356.
[14] Eseobar M A,Civerolo E L,Sumraeffeh K R,et al.RNAimediated oncogene silencing Colzfers resistance to crown gall tumorigenesis[J].Proc Nail Aead Sci USA,2001,98(23):13437-3442.

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