Construction of Expression Vector for Anti Stripe Virus of Rice by RNA Interference

doi:10.7668/hbnxb.2008.04.003

Abstract

Abstract: By using the sequence of stripe virus published on line and the software of siRNA Target Finder, we ob - tained 168 siRNA segments.After BLAST Scan of rice genome, 6 segments were selected and 2 were chemically synthe - sized and used to construct of expression vector ( pASV1301 -1, pASV1301 -2) intergratedwith pCAMBIA1301 plasmid.The recombined plasmid was successfully transformed into agrobacterium of EHA105,which lay a foundation for the transfor - mation of rice and the prevention of stripe virus disease of rice by RNA interference.

Key words: Rice, RNA interference, Construction of expression vector, Ant- i virus

CLC Number:

S432.4+1

ZHANG Gong, LIU Li-feng, ZHOU Wei, WANG Hai-guang, MA Zhi-ying. Construction of Expression Vector for Anti Stripe Virus of Rice by RNA Interference[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 10-13. doi: 10.7668/hbnxb.2008.04.003.

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