Isolation of gma-miR160o Promoter from Glycine max and Construction of Its Plant Expression Vectors

doi:10.7668/hbnxb.2015.01.004

Abstract

Abstract: In order to investigate the regulation mechanism of miRNA expression, in this study, based on the predicted sequence of gma-miR160o promoter region, primers of promoter sequences were designed.A 1 910 bp fragment was cloned from the genomic DNA of the soybean Williams 82 by PCR method.Promoter sequence analyzed by the database of PlantCARE and PLACE showed that the sequence contained basic elements TATA-box, CAAT-box and some cis -acting elements involved in response to abiotic stresses, light and plant hormones.The genomic DNA fragment in gma-miR160o promoter and GUS fusion expression vector were constructed by replacing CaMV35S promoter of pBI121.

Key words: Soybean, gma-miR160o, Promoter, Plant expression vector

CLC Number:

Q78
S565.03

NI Zhi-yong, YU Yue-hua, LIU Chao, REN Yan-ping, CHEN Quan-jia, QU Yan-ying. Isolation of gma-miR160o Promoter from Glycine max and Construction of Its Plant Expression Vectors[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 19-23. doi: 10.7668/hbnxb.2015.01.004.

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http://www.hbnxb.net/EN/Y2015/V30/I1/19

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