Effect of Sulfated Yeast β-glucan on Hemagglutination and mRNA Transcription of H1N1 Swine Influenza Virus in vitro

doi:10.7668/hbnxb.2015.01.003

Abstract

Abstract: To study the effect of sulfated yeast β-glucan on mRNA transcription and hemagglutination of H1N1 swine influenza virus in vitro, influenza virus infected MDCK was taken as model, SYBR Green Real-time PCR and HI test was replication adopted to measure the effect of sulfated yeast β-glucan on mRNA transcription and hemagglutination.The results showed that the sulfated β-glucan could inhabit the hemagglutination at the concentration of over 1.25 mg/mL, while it could inhibit influenza virus's mRNA transcription at the concentration of 5 mg/mL.Sulfated yeast β-glucan had the ability to inhabit reproduction of H1N1 SIV in vitro, and the ability to inhabit hemagglutination and mRNA transcription may be targets of sulfated yeast β-glucan to anti-SIV.

Key words: Sulfated β-glucan, H1N1 SIV, Hemagglutination, mRNA transcription

CLC Number:

S852.65

SUN Ying-feng, WANG Li-li, LU Chao, LI Xiu-li, ZHANG Li, YANG Chun-lei, FENG Jing, REN Wei-ke, TIAN Xiang-xue. Effect of Sulfated Yeast β-glucan on Hemagglutination and mRNA Transcription of H1N1 Swine Influenza Virus in vitro[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 15-18. doi: 10.7668/hbnxb.2015.01.003.

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