Purification of Porcine Circovirus-like Agent Pl with Immuno-affinity Chromatography

doi:10.7668/hbnxb.2013.S1.076

ACTA AGRICULTURAE BOREALI-SINICA ›› 2013, Vol. 28 ›› Issue (S1): 397-399. doi: 10.7668/hbnxb.2013.S1.076

Special Issue: Animal husbandry；

Previous Articles Next Articles

Purification of Porcine Circovirus-like Agent Pl with Immuno-affinity Chromatography

WEN Li-bin¹, HE Kong-wang¹, XIE Jian-ping¹, YANG Hao², ZHOU Jian-xin², JIN Quan-sheng², WANG Yu-ran²

1. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014;
2. Hebei Animal Health Instpection, Shijiazhuang 050081, China

Received:2013-07-30 Published:2013-12-31

Abstract

Abstract: This study deals mainly with porcine circovirus}ike agnet Pl antigen purified by immuno affinity chromatography. The purified Pl virus, which came from elution buffers from adsorption column coupled antill epitope-ontaining peptide antibody to either Epoxy}etivated agarose or NHS activated agarose,was confirmed by transmission electron microscopic observations. The study suggests that purified Pl antigen by immuno-ffinity chromatography is an effective way.

Key words: Porcine circovirus-like went P1, Purification, Immuno-affinity chromatography

CLC Number:

S852.65

WEN Li-bin, HE Kong-wang, XIE Jian-ping, YANG Hao, ZHOU Jian-xin, JIN Quan-sheng, WANG Yu-ran. Purification of Porcine Circovirus-like Agent Pl with Immuno-affinity Chromatography[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(S1): 397-399. doi: 10.7668/hbnxb.2013.S1.076.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2013.S1.076

http://www.hbnxb.net/EN/Y2013/V28/IS1/397

[1]	FAN Huifen, SUN Tianjie, SU Weihua, XIAO Fuming, ZHANG Jie, WANG Dongmei. Preparation of GmWRKY50 Protein Antibody and Analysis of Its Protein Level Expression [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 28-34.
[2]	LEI Qidong, SUN Xudong, XU Huini. *Cloning and Prokaryotic Expression of Arabidopsis AtTCP4* Gene** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 24-28.
[3]	JIANG Nan, ZHENG Qianqian, LI Qianwen, TU Jian, SONG Xiangjun, SHAO Ying, LIU Hongmei, QI Kezong. Prokaryotic Expression Vector Construction,Expression,Purification and Identification of the APEC Virulence Gene ygeG [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 184-190.
[4]	ZHENG Yichao, ZHANG Hao, ZHAO Dan, WANG Fengru, LIU Xigang, GUO Lin. Purification and Function Exploration of Transcription Factor MYB54 in Arabidopsis thaliana [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 47-55.
[5]	SUN Lijing, LI Qianying, WANG Peinan, SUN Ying. Protein Expression and Purification of OsAHP2 in Rice [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 46-51.
[6]	WANG Min, LIU Yuanyuan, ZHOU Fan, JIANG Tingting, ZHENG Xu, ZHANG Jing, SHI Cuiping, XING Jihong, DONG Jingao. Prokaryotic Expression Analysis of BcKMO Gene from Botrytis cinerea [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(1): 68-72.
[7]	ZHANG Feiyan, WANG Zhenhua, PAN Kangcheng, TANG Huiqin, GU Xiaoxiao, LI Wei. Cloning of Tibetan Pig IGF-1 Mature Peptide Gene and Its Prokaryotic Expression [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(1): 80-85.
[8]	LEI Haiying, BAI Fenglin, LIU Jianxia, WANG Zhijun. *Cloning,Expression and Bioinformatics Analysis of Maize Zmcen* Gene** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 18-24.
[9]	WANG Xiaoye, SHI Bomei, WANG Yingqun, LI Xun, LIU Deyu, LI Ming, LI Fangfang, HU Chuanhuo. Prokaryotic Expression Plasmid Construction and Expression/Purification of Anti-apoptotic Fusion Protein PTD-Bcl-xL [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(1): 1-7.
[10]	GUO Ziping, ZHAI Qinghua, ZENG Lingfeng, DENG Xiping, YANG Shushen. *Construction of TaGAPC* Site-directed Mutagenesis Gene Vector and Prokaryotic Expression Analysis from Wheat** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(1): 46-50.
[11]	ZHANG Meng, WAN Xiu-qing, QIAO Chan, LI Ruo, LI Xiu-lan, SUN Jian-ping, GUO Zhao-kui. Isolation and Purification of Tobacco Wildfire Bacteria Using PCR Technique [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 329-332.
[12]	YANG Ting, LIAO Mei-de, HE Yu-guang, LIU Si-jia. *Preliminary Study on the Factor of Promoting Plant Growth from Paecilomyces lilacinus* PL-HN-16** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(6): 170-175.
[13]	ZHU Xin-chan, WANG Qian-wen, YU Qun. Isolating and Bio-characterization of Chitinase from Momordica charantia [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(6): 182-187.
[14]	DING Qiang, ZHANG Li-ping, JIA Jian-lei, WANG Jing, ZHANG Yong. *Prokaryotic Expression of FecB* Gene and Generation of Its Polyclonal Antibody** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(3): 31-36.
[15]	XIE Wei-yi, BI Yan-hui, ZHOU Zhi-gang. Prokaryotic Expression and Purification of Nulear-genomic cbbX Gene of Saccharina japonica [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 64-71.

Please choose a citation manager

Content to export

Purification of Porcine Circovirus-like Agent Pl with Immuno-affinity Chromatography

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

模态框（Modal）标题

Please choose a citation manager

Content to export

Purification of Porcine Circovirus-like Agent Pl with Immuno-affinity Chromatography

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments