Distribution and Expression of Melatonin Receptor Mel1b in Different Tissues of Wanxi White Geese

doi:10.7668/hbnxb.20194249

Abstract

Abstract:

Melatonin is an important neurohormone in animals,which exerts a variety of biological effects by binding to melatonin receptors.The purpose of this study was to investigate the distribution characteristics and expression levels of melatonin receptor Mel1b in different tissues of Wanxi white geese.The heart,liver,spleen,lung,kidney,brain,cerebellum,pancreas,pectoral muscle,ovary and ovarian hierarchical follicles of Wanxi white geese were collected.The distribution characteristics and expression levels of Mel1b in various tissues were detected by immunohistochemistry and Real-time PCR.The results showed that Mel1b was widely distributed in the heart,liver,spleen,lung,kidney,brain,pancreas,pectoral muscle,ovary and ovarian hierarchical follicles of Wanxi white goose,and there were some differences in the expression levels of Mel1b gene and protein among different tissues.The expression level in ovary was the highest,and the expression level gradually increased with the development of follicles,followed by pancreas,spleen,kidney and lung,and the expression level in brain,heart,liver and pectoral muscle was the least.It could be seen that melatonin receptor Mel1b exists in all tissues of Wanxi white goose,and the expression levels were different among different tissues.The results of this study lay a theoretical foundation for further exploring the involvement of melatonin and its receptors in the regulation of a wide range of biological functions in Wanxi white goose.

Key words: Wanxi white goose, Melatonin receptor, Mel1b, Expression distribution

ZHENG Menghao, LI Rui, LIU Yanyan, CHEN Zhihao, WANG Jiaqi, PANG Xunsheng, LIU Wenju, WANG Shujuan. Distribution and Expression of Melatonin Receptor Mel1b in Different Tissues of Wanxi White Geese[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 423-428. doi: 10.7668/hbnxb.20194249.

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Figures/Tables 4

Tab.1 Primers used in present study

基因 Genes	序列(5'—3') Sequence(5'—3')	产物大小/bp Product size	退火温度/℃ Annealing temperature
Mel1b	TGTGGTAATTCATTTCATCGTCCC	239	61
	TTGGTGCCATTTCTGAAGGATTGAT
GAPDH	GTGGTGCAAGAGGCATTGCTGAC	86	60
	GCTGATGCTCCCATGTTCGTGAT

Fig.1 Expression of Mel1b gene in goose tissue M.Marker;1.Cerebral;2.Cerebellum;3.Lung;4.Pectoral muscle;5.Pancreas;6.Spleen;7.Heart;8.Kidney;9.Liver;10.Ovary;11.F1;12.F2;13.F3;14.F4;15.F5.

Fig.2 Distribution of Mel1b protein in goose tissue A.Distribution of Mel1b protein in tissues;B.IOD value of Mel1b-positive signal in each tissue.The same superscript letter indicates a non-significant difference (P<0.05) and different indicates a significant difference (P>0.05).The same as Fig.3.

Fig.3 Differential expression of Mel1b mRNA and protein in various tissues of geese A.Relative expression levels of Mel1b mRNA in each tissue;B.Relative expression levels of Mel1b mRNA in graded follicles;C.Mel1b protein expression levels in each tissue.

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