Abstract: Wheat substitution lines between Chinese Spring and Synthetic 6x under the treatments of salt stress were studied to research the effect on antioxidant enzymes SOD,POD activities and MDA content and locating the gene controlling antioxidant enzymes SOD,POD activities and MDA content.Two control groups were carried out under hydroponic experiment.The first control group was growing under Hoagland's solution(0 mmol/L NaCl).The second control group was growing under Hoagland's solution with 150 mmol/L NaCl.Seedlings was treated at the two-leaf stage and measured SOD and POD activity and MDA content under control and salt treatment conditions at four-leaf stage.Substitution lines of wheat significantly increased of SOD and POD activity under salt stress conditions,but the MDA content decreased.SOD activity of 1A,5A,6A,1B,5B,6B,7B and 5D substitution lines were significantly or very significantly higher than female Chinese Spring.2A,1B,2B,3B,5B,6B,5D and 6D substitution lines of relative SOD activity was significantly or very significantly higher than female Chinese Spring;POD activity of 3A,4A,5A,6A,7A,6B and 7D substitution lines were significantly or very significantly higher than China Spring,4A,5A,6B,1D and 7D substitution lines of relative POD activity significantly or very significantly higher than Chinese Spring.As the role of protective enzymes under salt stress conditions,the MDA content of substitution lines was significantly reduced.2A,3A,4A,6A,1B,3B,4B,5B,6B,7B,1D,5D and 7D substitution lines of MDA content were significantly or very significantly lower than female Chinese Spring,2A,6A,6B,1D and 2D substitution lines of relative MDA content significantly or very significantly lower than female Chinese Spring.The results showed that the genes increased SOD activity and POD activity might be located on 1B,5B,6B and 5D chromosome and 4A,5A,6B and 7D chromosome of Synthetic 6x respectively;while the genes inhibiting content might be exist in 2A,6A,6B and 1D chromosome of Synthetic 6x under salt stress.

Key words: Substitution lines, Salt stress, Protective enzymes, Chromosome effect

CLC Number: 

• S512.01