Functional Study of TaGAMyb-B Conferring Wheat Plant Height in Transgenic Rice

doi:10.7668/hbnxb.20193241

Abstract

Abstract:

To further investigated the effect of different allelic variants of TaGAMyb-B genes in wheat on stem elongation, used the rice Agrobacterium transformation system,RT-qPCR,tissue section and cell tissue specific analysis to systematically study the function of the 84 bp InDel of TaGAMyb-B.The results showed that,in the over-expressed transgenic rice lines,transcript expression of TaGAMyb-B was detected in seeds,roots,stems and leaves;TaGAMyb-Bb-GFP transgenic seeds were more sensitive under the treatment of NaCl,GA and mannitol compared with TaGAMyb-Ba-GFP;the diameters of first,second and third stem inter-nodes,spike length and tiller number of TaGAMyb-Ba-GFP were significantly larger than that of TaGAMyb-Bb-GFP;and the analysis of cell tissue sections showed that the average thickness of the thick-walled tissue cells in the transverse sections of the transgenic TaGAMyb-Ba-GFP rice was significantly greater than that of the transgenic TaGAMyb-Bb-GFP event,the average length of thick-walled cells was extremely significantly shorter than that of transgenic TaGAMyb-Bb-GFP event.The above mentioned results indicated that the 84 bp deletion in TaGAMyb-B increased not only abiotic stress resistance and plant lodging resistance,but also spike length and tiller number in transgenic rice.

Key words: Transgenic rice, TaGAMyb-B, Allelic variation, Abiotic stress, Lodging resistance

LIU Juan, FENG Yumei, HAN Bing, XING Yanping, LI Shufen, YANG Yan. Functional Study of TaGAMyb-B Conferring Wheat Plant Height in Transgenic Rice[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(3): 42-48. doi: 10.7668/hbnxb.20193241.

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Figures/Tables 11

Tab.1 The primer sequences used in this study

引物名称 Primer name	上游引物(5'-3') Forward primer(5'-3')	下游引物(5'-3') Reverse primer(5'-3')	退火温度/℃ TM	片段大小/bp Fragment size
GAMyb-B2F/R	CTACAGATTTTTATTTTTATCTATC	CAACAAGAGGTGAACTTTTTT	57.0	659/764
JDF/R	ACTTGTTTGTCGGGTCATCTTTTC	GAAGTAAAGCAGTGAATCAGCGTG	60.0	1 106
Q-OsActinF/R	CCTGACGGAGCGTGGTTAC	CCAGGGCGATGTAGGAAAGC	63.0	321
Q-TaGAMyb-BF/R	GCGAGAATCTTTCCAGTGACCTT	CAATCCAGGGAGTAAATGGTCAGA	60.0	230

Fig.1 Identification of the recombinant expression vector by restriction endonucleases

Fig.2 Identification of the recombinant expression vector(Ubi-TGAMyb-Ba/b-GFP)by PCR

Fig.3 Identification of positive transgenic rice lines by PCR M.DL2000 bp DNA Marker;1-9.Some positive individuals of transgenic rice; CK.Negative individuals of transgenic rice(no gene for this test).

Fig.4 The relative expression levels of TaGAMyb-B in transgenic rice

Fig.5 Expression changes of TaGAMyb-B in transgenic rice with during different stress treatments

Fig.6 Statistic and analysis of phenotype in transgenic rice Different lowercase letters indicate significant different,P<0.05.The same as Fig.8.

Fig.7 Transverse sections of second stem section in transgenic rice

Fig.8 Average thickness of sclerenchyma in transverse sections of second stem section in transgenic rice

Fig.9 Longitudinal sections of second stem section in transgenic rice

Fig.10 Average length of cell in longitudinal sections of second stem section in transgenic rice Different capital letters indicate extremely significant,P<0.01.

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