The Application of PCR in Detecting for Aquatic Animal’s Pathogentic Microorganism.

doi:10.7668/hbnxb.2007.S3.024

Abstract

Abstract: Withing the elevation of aquaculture scale in our country, Shrimp, shellfish, and fish farming suffered the serious efG",ct from pathogentic microorganism. So how to detect aqu.aic animal's disease lastly isvery important and exserted problem for aquaculture. Recently years, with the intervention of PCR, spuring thedetection technique of Aquatic Animal's pathogentic microorganism fast development. This article reviews theprinciples,characteristics and application of PCR and its current derivative techniques, RT-PCR,Realtime-PCR,Immunity -PCR,Nested primer -PCR,Multiplex -PCR in detecting for aquatic animal's pathogenticmic roorganism and make a prospect for the development of aquatic animal's pathogenic microorganisms diagnostic techniques.

Key words: PCR, Pathogenic microorganisms, Aquaculture, Derivative techniques

CLC Number:

LI ZHi-ming, FENG Wei-ye. The Application of PCR in Detecting for Aquatic Animal’s Pathogentic Microorganism.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(S3): 98-101. doi: 10.7668/hbnxb.2007.S3.024.

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