Subcellular Localization of ZmSPK1, A Protein Kinase from Maize, in Transgenic Arabidopsis

doi:10.3321/j.issn:1000-7091.2006.03.001

Abstract

Abstract: To determine the subcellular localization of ZmSPK1,a protein kinase from maize,using 35S promoter we constructed two plant expression plastids:35S::ZmSPK1GFP and 35S::GFP,and transformed them respectively into Arabidopsis by floral dip.Subcellular localization was determined by Confocal laser scanning microscopy.The fused ZmSPK1-GFP showed a nuclear and cytosolic distribution similar to that of free GFP.Compared with 35S::GFP,ZmSPK1GFP was more strongly accumulated in the nucleus,suggesting that ZmSPK1 might be soluble protein and localize in the nucleus.

Key words: ZmSPK1, GFP, Confocal laser scanning microscope (CLSM), Subcellular localization

CLC Number:

S188

XIAO Xue, ZHANG Xiu-hai, YANG Qing, ZOU Hua-wen, WU Zhong-yi, YU Rong, CAO Ming-qing, HUANG Cong-lin. Subcellular Localization of ZmSPK1, A Protein Kinase from Maize, in Transgenic Arabidopsis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(3): 1-4. doi: 10.3321/j.issn:1000-7091.2006.03.001.

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