Molecular Check and Measure of Transgenic Maize Lines Resistancing to Maize Head Smut and Breeding Selection for Transgenic Lines Resistancing to Disease

doi:10.3321/j.issn:1000-7091.2006.05.024

Abstract

Abstract: In this study 128 transgenic maize seeds that were obtained from transgenic plants transformed by pollen-mediated approach on maize(Zea mays L.) inbred line Hai 92-1,the vector was plasmid pGL II-RC-1.The result of seeds selected by hygromycin-screening had proved that 16 plants was hygromycin-resistant plants.Statistics analysis for the hygromycin-resistant plants suggested that single copy of the chintinase gene was transferred into the cell of majority of T-(0) transgenic plants,segregation ratio of the progeny of transgenic plants for hygromycin resistant and susceptible was about 3:1,P<0.01.The results of detecting to T-1 transgenic plants by PCR amplification and PCR Southern blot hybridization showed that chitinase gene was transferred into the cells of transgenic plants.The same methods detected to T₂ and T₃,their results indicated that chitinase gene was integrated into the genome of transgenic plants and stable inheritance in transgenic plants and their progeny.The results of Bio-assay for head smut-resistance activity indicated that the disease incidence of transgenic plants and their progeny was lower than that of control plants.Based on the results above,GH05024 and GH05028.2 transgenic maize lines that resisted to maize head smut were obtanined by breeding selection.

Key words: Maize, Chitinase gene, Transgene, Maize head smut, Breeding selection

CLC Number:

S435.131.4+2

DU Jian-zhong, SUN Yi, HAO Yao-shan, LIU Jun-ling. Molecular Check and Measure of Transgenic Maize Lines Resistancing to Maize Head Smut and Breeding Selection for Transgenic Lines Resistancing to Disease[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(5): 99-104. doi: 10.3321/j.issn:1000-7091.2006.05.024.

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References

[1] 王振华,姜艳喜,王立丰,等.玉米丝黑穗病的研究进展[J].玉米科学2002,10(4):61-64.
[2] 侯文邦,朱文文,马占强.植物基因工程在作物育种中的应用与展望[J].中国农学通讯,2005,21(1):128-132.
[3] broglie K J,Chet L,Holliday M,et al.Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia[J].American Association for the Advancement of Science,1991,254(5035):1194-1197.
[4] 杨崇良,路兴波,张君亭.世界农业转基因生物、产品研发及其安全性监管[J].山东农业科学2005,(2):66-68.
[5] 郝转芳,毛雪,李润植.转基因改良植物抗真菌病害的策略及其进展[J].生物技术通报,2001,(6):9-11.
[6] WANG Jing-xue,SUN Yi,CUI Gui-mei,et al.Transgenic maize plants obtained by pollen-mediated transformation[J].Acta Botanica Sinica,2001,43(3):275-279.
[7] 胡适宜.植物胚胎学实验方法(一):花粉生活力的测定[J].植物学通报,1993,10(2):60-62.
[8] 林良斌,官春云,周小云,等.转基因抗虫油菜中Bt杀虫蛋白基因稳定遗传和高效表达及抗虫性研究[J].作物学报,2002,28(2):175-178.
[9] 杨立国,石太渊,林风,等.生物技术在玉米育种中的应用[J].辽宁农业科学2000,(4):28-29.
[10] Maliga P,Graham I.Plants biotechnology:Molecular farming and metabolic engineering promise a new generation of hightech crops[J].Current Opinion in Plant Biology,2004,7(2):149-153.
[11] LI X B,Mao H Z,BAI Y Y.Transgenic plants of rutabzgz(brassica Napobrassica) tolerant to pest insects[J].Plant Cell Reports,1995,15(1-2):97-101.
[12] 毛慧珠,唐惕,曹湘玲,等.抗虫转基因甘蓝及后代的研究[J].中国科学C辑:生命科学1996,26(4):339-347.
[13] 李学宝,秦明辉,施荣华,等.芥菜型油菜抗虫转基因植株及其后代株系的研究[J].生物工程学报,15(4):482-488.
[14] Matzke J M,Matzke A M.Position effects and eqigenetic silencing of plant transgenes[J].Current Opinion in Plant Biology,1998,1 (2):142-148.
[15] Dieguez M J,Vaucheret H,Paszkowski H J,et al.Cytosine methylation at CG and CNG sites is not a prerequisite for the initiation of transcriptional gene silencing in plants,but it is required for its maintenance[J].Molecular & General Genetics,1998,259(2):207-215.

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