Cloning and Analysis of Chitinase Gene IFCHI1 from Egg-parasitic Fungus Isaria farinosa

doi:10.7668/hbnxb.2015.02.015

Abstract

Abstract: In order to explore the function of chitinase in parasitizing and destroying egg, a nematode egg-paraastic fungus Isaria farinosa HNE3 was isolated from Meloidogyne incognita infecting tobacco in Henan Province.By using RT-PCR and RACE, we had cloned a chitinase gene IFCHI1 for the first time.The full-length DNA of IFCHI1 was 1 417 bp, containing a intron with the length of 61 bp, ORF was 1 185 bp and it encoded 394 amino acids.Analyzing chitinase IFCHI1 by online biological software deduced that its molecular mass and isoelecftric point were 44.1 kDa and 4.88, especially.By compared with other chitinase from different fungus in GenBank, IFCHI1 belongs to glycosyl hydrolase family 18 chitinase.We found two prevalent conserved catalytic domains with the sequence of SIGGW and FDGIDIDWE and two binding domains with the sequence of GTWEQGVYDY and GLGGAMWWESS.Comparison of chitinase IFCHI1 with other chitinases from entomopathogenic fungi revealed that enzymes were highly similar.It was concluded that the chitinase coded by gene IFCHI1 provided theoretical basis for damaging on eggshell structure and inhibiting on egg-hatching of M.incognita.

Key words: Isaria farinosa, Meloidogyne incognita, Cloning, Chitinase gene, Sequence analysis

CLC Number:

WANG Kai, ZHAO Yang, ZHU Xian-ting, CAI Xiu-hua, WU Xia. Cloning and Analysis of Chitinase Gene IFCHI1 from Egg-parasitic Fungus Isaria farinosa[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 78-86. doi: 10.7668/hbnxb.2015.02.015.

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