Abstract: The aim of the study to investigate the main structural protein of the Kobuvirus VP1 gene.According to the sequences of PKV deposited in GenBank, a pair of special primers was designed for amplifying the VP1 gene of swKoV CH441 strain by RT-PCR.The results of sequence analysis showed that the whole VP1 gene of swKoV CH441 strain consisted of 762 bp.Compared with 15 PKV strains which were deposited in GenBank, the homology of nucleotide sequences was 81.5% ~90.2%, and the homology of deduced amino acids was 86.6% ~96.9%.Evolution analysis indicated that the swKoV CH441 strain was closely related to GS-1 strains.The bioinformatics analysis demonstrated that the isoelectric point and molecular weight of non-structural protein VP1 were 4.40 and 26.978 2 kDa.The protein had no signal peptide and transmembrane domain.There were 18 phosphorylation sites including 7 Sers, 6 Thrs and 5 Tyrs.Protein phosphorylation was concerned with signal transduction, so this protein may be a signaling molecule.The results provided a theoretical foundation for further research on the study of VP1 gene (protein) in the genetic variation.

Key words: VP1 gene, Cloning, DNA sequencing, Sequence analysis

CLC Number: 

• Q78

• S828