Comparing Analysis of Diversity of Chinese Leek Rhizospheritic and Endophytic Fungal Communities

doi:10.7668/hbnxb.2018.S1.039

Abstract

Abstract: In the preliminary study,the incidence of Fusarium wilt of banana and the disease caused by root-knot nematode were significantly reduced by Chinese leek. It was deduced that some potential biological fungus with antifungal and nemotocidal activity existed in the Chinese leek. In the present study the rhizospheric and endophytic fungus communities and diversities were comparatively analyzed,aiming to provide theoretical basis for isolating and identifying biological fungus with antifungal and nematocidal activity in Chinese leek. 18S rRNA from the samples of Chinese leek rhizosphere soil,leave and root were sequenced on the Illumina Miseq platform,and the datas were comparatively analyzed using bioinformatics. The results showed a total of 348 484 high quality sequences and 123 operational taxonomic units(OTUs) was obtained from the Chinese leek rhizosphere siol, leave and root. The Chao,ACE,Shannon index of rhizospheric fungus were significantly higher and Simpson index was significantly lower than that of endophytic fungus(leave and root). The rhizopheric fungus was mainly composed by Ascomycota(67.20%) and Basidiomycota(24.44%). The endophytic fungus was mainly composed by Ascomycota,91.39% in leave and 96.60% in root. In the identified five fungus phyla,the amount of Ascomycota in endophytic fungus was significantly higher than that in the rhizophytic fungus,the other fungus phyla was significantly lower than that in the rhizophytic fungus. The fungus community in the leave and root was slightly different,but not significant. The present study showed significantly difference in the rhizophytic and endophytic fungus communities. The diversity and richness of rhizophytic fungus were significantly higher than that of endophytic fungus. The findings of the study provide reference to isolate biological fungus with potential antifungal and nematocidal activity from Chinese leek.

Key words: Chinese leek, Fungal diversity, Illumina sequence, Endophyte, Rhizophyere soil

CLC Number:

S432

FU Jing, LIU Yanfeng, MEI Mei, HUANG Yonghong. Comparing Analysis of Diversity of Chinese Leek Rhizospheritic and Endophytic Fungal Communities[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(S1): 246-252. doi: 10.7668/hbnxb.2018.S1.039.

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