Identification of Antagonistic Endophytic Bacterium YB-144 Strain from Fusarium Head Blight Against and Its Effect on DON

doi:10.7668/hbnxb.20191619

Abstract

Abstract: In order to study the safe and effective biological control technology to control the growth of Fusarium graminearum and inhibition its of production, 256 strains of endophytic bacteria were isolated from surface sterilized spikes of wheat with scab symptoms by plant endophyte isolation technology. Of them,a strain of antagonistic endophytic bacteria YB-144, which could significantly inhibit the growth of Fusarium graminearum and decrease DON content, was screened through the plate confrontation and co culture experiments.Strain YB-144 was identified as Bacillus velezensis based on morphological characteristics, biochemical characteristics and 16S rDNA gene sequence analysis.The inhibitory band width of the strain YB-144 against Fusarium graminearum PH-1 was 13.6 mm. After 15 h of co-culture, the inhibition rate of spore germination was as high as 81.67%, and the content of DON decreased by 66.6%. The expression levels of DON synthetic genes including Tri5, Tri3 and Tri8 were down-regulated by 5.0, 1.6 and 1.2 times, respectively. In addition, strain YB-144 displayed the antagonist effects against other 9 different plant pathogens, such as Rhizoctoniasolani cerealis and Bipolaris sorokiniana et al. The above results demonstrated that strain YB-144 has good potentials of exploitation and utilization.

Key words: Fusarium head blight, Fusarium graminearum, Bacillus velezensis, DON, Endophytic bacteria

CLC Number:

S435.121

ZHANG Liyong, XIA Mingcong, XU Wen, WANG Qi, ZHANG Jie, SUN Runhong, PAN Yamei, CHEN Ruixue, WU Kun, YANG Lirong. Identification of Antagonistic Endophytic Bacterium YB-144 Strain from Fusarium Head Blight Against and Its Effect on DON[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 172-179. doi: 10.7668/hbnxb.20191619.

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References

[1] McMullen M, Jones R, Gallenberg D. Scab of wheat and barley:A re-emerging disease of devastating impact[J]. Plant disease, 1997, 81(12):1340-1348.doi:10.1094/PDIS.1997.81.12.1340.
[2] Kang Z S, Buchenuer H, Huang L L, Han Q M, Zhang H C. Cytological and immunocyto-chemical studies on responses of wheat spikes of the resistant Chinese cv. Sumai 3 and the susceptible cv. Xiaoyan 22 to infection by Fusarium graminearum[J]. European Journal of Plant Pathology, 2007, 120(4):383-396.doi:10.1007/s10658-007-9230-9.
[3] Jiang C, Zhang C K, Wu C L, Sun P P, Hou R, Liu H Q, Wang C F, Xu J R. TRI6 and TRI10 play different roles in the regulation of deoxynivalenol(DON) production by cAMP signalling in Fusarium graminearum[J]. Environmental Microbiology, 2016, 18(11):3689-3701.doi:10.1111/1462-2920.13279.
[4] Tralamazza S M, Bemvenuti R H, Zorzete P, Garcia F S, Corrêa B. Fungal diversity and natural occurrence of deoxynivalenol and zearalenone in freshly harvested wheat grains from Brazil[J]. Food Chemistry, 2016, 196:445-450.doi:10.1016/j.foodchem.2015.09.063.
[5] Zhang J B, Wang J H, Gong A D, Chen F F, Song B, Li X, Li H P, Peng C H, Liao Y C. Natural occurrence of Fusarium head blight, mycotoxins and mycotoxin-producing isolates of Fusarium in commercial fields of wheat in Hubei[J]. Plant Pathology, 2013, 62(1):92-102.doi:10.1111/j.1365-3059.2012.02639.x.
[6] 程志伟. 小麦赤霉病防治误区及综合防控技术[J].种业导刊, 2017(3):24-25.doi:10.3969/j.issn.1003-4749.2017.03.010.
Cheng Z W. Misunderstanding and integrated control technology of wheat scab[J]. Seed Industry Guide, 2017(3):24-25.
[7] 杜晓昱. 禾谷镰孢产毒分析及其毒素检测方法的研究[D]. 福州:福建农林大学, 2005.doi:10.7666/d.y775052.
Du X Y. Study on analysis of Fusarium graminearum toxin production and detection method of its toxin[D].Fuzhou:Fujian Agriculture and Forestry University, 2005.
[8] Bai G H, Gregory S. Management and resistance in wheat and barley to Fusarium head blight[J]. Annual Review of Phytopathology, 2004, 42(1):135-161.doi:10.1146/annurev.phyto.42.040803.140340.
[9] Xiong K H, Hu W, Wang M J, Wei H, Chen B C. A survey on contamination of deoxynivalenol and zearalenol in maize and wheat from Anhui and Henan Province[J]. Food Science, 2009, 30(20):265-268.doi:10.7506/spkx1002-6300-200920056.
[10] 何慧慧, 李力, 韩小贤, 郑学玲, 卞科. 河南省市售小麦粉微生物及真菌毒素污染状况研究[J].粮食与油脂, 2015, 28(12):64-66.doi:10.3969/j.issn.1008-9578.2015.12.018.
He H H, Li L, Han X X, Zheng X L, Bian K. Study on microorganism and mycotoxins contamination situation of commercial wheat flour in Henan Province[J]. Cereals and Oils, 2015, 28(12):64-66.
[11] Miedaner T, Korzun V. Marker-assisted selection for disease resistance in wheat and barley breeding[J]. Phytopathology, 2012, 102(6):560-566.doi:10.1094/PHYTO-05-11-0157.
[12] 刘昆昂, 黄亚丽, 贾振华, 马宏, 宋水山. 拮抗灰霉病菌的芽孢杆菌筛选、鉴定及其代谢产物抑菌效果研究[J].华北农学报, 2020, 35(3):200-207.doi:10.7668/hbnxb.20190756.
Liu K A, Huang Y L, Jia Z H, Ma H, Song S S. Screening and identification of Bacillus sp. and its metabolites inhibitory effect against Botrytis cinerea[J]. Acta Agriculturae Boreali-Sinica, 2020, 35(3):200-207.
[13] 邹文欣,谭仁祥.植物内生菌研究新进展[J].植物学报,2001,43(9):881-892.doi:10.3321/j.issn:1672-9072.2001.09.001.
Zou W X, Tan R X.Recent advances on endophyte research[J]. Acta Botanica Sinica, 2001,43(9):881-892.
[14] 周清, 舒宪, 王钰婷, 李明浩, 汤才国, 倪军, 赵薇薇, 黄胜威, 吴丽芳. 一株小麦赤霉病拮抗菌的筛选鉴定及防治效果分析[J].生态科学, 2019, 38(3):64-70.doi:10.14108/j.cnki.1008-8873.2019.03.008.
Zhou Q, Shu X, Wang Y T, Li M H, Tang C G, Ni J, Zhao W W, Huang S W, Wu L F. Screening of antagonistic strain against Fusarium head blight and its inhibition efficiency[J]. Ecological Science, 2019, 38(3):64-70.
[15] 杨凤, 张玲利, 王东梅, 刘燕, 周敏, 张国林, 代玉梅, 罗应刚. 小麦内生菌Pedobacter chitinilyticus CM134L-2^T的次级代谢产物分离与鉴定[J].应用与环境生物学报, 2020, 26(4):1-7.doi:10.19675/j.cnki.1006-687x.2019.10017.
Yang F, Zhang L L, Wang D M, Liu Y, Zhou M, Zhang G L, Dai Y M, Luo Y G. Isolation and identification of secondary metabolites of endophytic bacteria Pedobacter chitinilyticus CM134L-2^T from wheat[J]. Journal of Applied and Environmental Biology, 2020, 26(4):1-7.
[16] R E布坎南, N E吉本斯.伯杰氏细菌鉴定手册[M].第8版. 北京:科学出版社,1984.
Buchanan R E, Gibbons N E. Berger's handbook of bacterial identification[M]. 8th ed. Beijing:Science Press, 1984.
[17] Yang L R, Xie L H, Xue B G, Goodwin P H, Quan X, Zheng C L, Liu T G, Lei Z S, Yang X J, Chao Y E, Wu C. Comparative transcriptom profiling of the early infection of wheat roots by Gaeumannomyces graminis var. tritici[J]. PLoS One, 2015, 10(4):1245-1264.doi:10.1371/journal.pone.0120691.
[18] 王亚君, 翟焕趁, 张帅兵, 刘新影, 蔡静平. 小麦赤霉病菌FgRab7调控Tri基因表达及DON毒素合成[J]. 河南工业大学学报(自然科学版), 2017, 38(2):57-62, 68.doi:10.16433/j.cnki.issn1673-2383.2017.02.010.
Wang Y J, Zhai H C, Zhang S B, Liu X Y, Cai J P. Regulation of Tri gene expression and don toxin synthesis by FgRab7 of Fusarium graminearum[J]. Journal of Henan University of Technology(Natural Science Edition), 2017, 38(2):57-62, 68.
[19] Jiang C, Zhang S J, Zhang Q, Tao Y, Wang C F, Xu J R. FgSKN7 and FgATF1 have overlapping functions in ascosporogenesis, pathogenesis and stress responses in Fusarium graminearum[J]. Environmental Microbiology, 2015, 17(4):1245-1260.doi:10. 1111/1462-2920.12561.
[20] Livak K J, Schmittgen T D. Analysis of relative gene expression data using Real-time quantitative PCR[J]. Methods, 2002, 25(4):402-408.doi:10.1006/meth.2001.1262.
[21] Tian Y, Tan Y L, Liu N, Liao Y C, Sun C P, Wang S X, Wu A B. Functional agents to biologically control deoxynivalenol contamination in cereal grains[J]. Frontiers in Microbiology, 2016(7):387-395.doi:10.3389/fmicb.2016.00395.
[22] Palazzini J M, Ramirez M L, Torres A M, Chulze S N. Potential biocontrol agents for Fusarium head blight and deoxynivalenol production in wheat[J]. Crop Protection, 2007, 26(11):1702-1710.doi:10.1016/j.cropro.2007.03.004.
[23] Wang L Y, Xie Y S, Cui Y Y, Xu J J, He W, Chen H G, Guo J H. Conjunctively screening of biocontrol agents(BCAs) against Fusarium root rot and Fusarium head blight caused by Fusarium graminearum[J]. Microbiological Research, 2015, 177:34-42.doi:10.1016/j.micres.2015.05.005.
[24] Johnson B A, Anker H, Meleney F L. Bacitracin:A new antibiotic produced by a member of the B.subtilis group[J]. Science, 1945, 102(2650):376-377.doi:10.1126/science.102.2650.376.
[25] Shi C J, Yan P S, Li J F, Wu H Q, Li Q W, Guan S S. Biocontrol of Fusarium graminearum growth and deoxynivalenol production in wheat kernels with bacterial antagonists[J]. International Journal of Environmental Research and Public Health, 2014, 11(1):1094-1105.doi:10.3390/ijerph110101094.
[26] 王瑶, 赵月菊, 邢福国, 王龑, 刘阳. 禾谷镰孢拮抗菌株的筛选及鉴定[J].核农学报, 2017, 31(6):1128-1136.doi:10.11869/j.issn.100-8551.2017.06.1128.
Wang Y,Zhao Y J, Xing F G, Wang Y, Liu Y. Screening and identification of Bacillus spp. strains with biocontrol activity against Fusarium graminearum[J]. Journal of Nuclear Agricultural Sciences, 2017, 31(6):1128-1136.
[27] He J, Boland G J, Zhou T. Concurrent selection for microbial suppression of Fusarium graminearum, Fusarium head blight and deoxynivalenol in wheat[J]. Journal of Applied Microbiology,2009,106(6):1805-1817.doi:10.1111/j.1365-2672.2009.04147.x.
[28] Schisler D A, Khan N I, Boehm M J, Slininger P J. Greenhouse and field evaluation of biological control of Fusarium head blight on durum wheat[J]. Plant Disease, 2002, 86(12):1350-1356.doi:10.1094/PDIS.2002.86.12.1350.
[29] 刘伟成, 潘洪玉, 席景会, 李宏宇, 郭永来, 王旭明. 小麦赤霉病拮抗性芽孢杆菌生防作用的研究[J].麦类作物学报, 2005,25(4):95-100.10.3969/j.issn.1009-1041.2005.04.020.
Liu W C, Pan H Y, Xi J H, Li H Y, Guo Y L, Wang X M. Biocontrol effects of some antifungal strains from Bucillus against pathogens of wheat head blight[J]. Journal of Wheat Crops, 2005,25(4):95-100.
[30] 周苗苗, 刘振林, 唐丽霖, 杨艺华, 杨继芝, 张敏, 陈华保, 杨春平. 小麦赤霉病菌拮抗内生菌的抑菌活性及鉴定[J].核农学报, 2016, 30(3):460-467.doi:10.11869/j.issn.100-8551.2016.03.0460.
Zhou M M, Liu Z L, Tang L L, Yang Y H, Yang J Z, Zhang M, Chen H B, Yang C P. Antifungal activity and identification of antagonistic endophytic on Fusarium graminearum[J]. Journal of Nuclear Agricultural Sciences, 2016, 30(3):460-467.
[31] González-Sánchez M Á, Pérez-Jiménez R M, Pliego C, Ramos C, De Vicente A, Cazorla F M. Biocontrol bacteria selected by a direct plant protection strategy against avocado white root rot show antagonism as a prevalent trait[J]. Journal of Applied Microbiology, 2010, 109(1):65-78.doi:10.1111/j.1365-2672.2009.04628.x.
[32] 胡晓丹, 王建伟, 李孝敬, 祭芳, 史建荣, 徐剑宏. 赤霉病菌拮抗菌Bacillus subtilis AF0907抗菌物质研究[J].中国生物防治学报, 2015, 31(3):378-385.doi:10.16409/j.cnki.2095-039x.2015.03.013.
Hu X D, Wang J W, Li X J, Ji F, Shi J R, Xu J H. Study of antagonistic substance from Bacillus subtilis AF0907 against Fusarium graminearum[J]. Chinese Journal of Biological Control, 2015, 31(3):378-385.

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