Construction of TALEN Genome-editing Technique for BGIOSGA032296 Related to Cold Tolerance in Rice Seedling Based on Comparative Transcriptome

doi:10.7668/hbnxb.2017.03.003

Abstract

Abstract: To dig cold tolerance genes at seedling stage of rice,based on the analysis of comparative transcriptome,TALEN genome-editing technique was constructed for BGIOSGA032296 which was down-regulated in Dongxiang wild rice seedling under cold stress.The analysis of enzyme identification and sequence alignment showed that TALEN knockout plant expression vector 1301TALEN-032296 was constructed successfully.By the agrobacterium-mediated method,the recombination clone 1301TALEN-032296 was transformed into the receptor rice variety TP309.17 transgenic rice plants were obtained.After PCR detection of hygromycin resistance gene and Fok I gene,they were found to be positive transgenic plants carrying 1301TALEN-032296.This research laid some basis for subsequent cold resistant phenotype identification.

Key words: Comparative transcriptome, Dongxiang wild rice, TALEN genome-editing technique

CLC Number:

Q78
S513.08

SHEN Chunxiu. Construction of TALEN Genome-editing Technique for BGIOSGA032296 Related to Cold Tolerance in Rice Seedling Based on Comparative Transcriptome[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(3): 13-20. doi: 10.7668/hbnxb.2017.03.003.

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