Cloning and Bioinformatics Analysis of StNF-Y Nuclear Transcription Factor Gene in Potato

doi:10.7668/hbnxb.201751585

Abstract

Abstract: To study the role and function of potato StNF-Y gene under abiotic stress, a new NF-YC transcription factor(StNF-Y) was cloned from potato resistant to bacterial wilt genotype ED13. Bioinformatics analysis and biological function prediction showed that the full-length cDNA of StNF-Y was 1 132 bp, with an open reading frame of 693 bp, encoding 230 amino acids, with a formula of C₁₁₁₂H₁₇₃₅N₃₁₇O₃₃₉S₁₀ and isoelectric point of 5.37. It contained 13 phosphorylation sites, and StNF-Y protein was distributed in the cytoplasm, without signal peptide.It was a hydrophilic protein. StNF-Y protein contained a highly conserved domain, existed in the BUR6 superfamily. Secondary structure showed that the protein consisted mainly of 45.65% α-helix, 5.65% extended chain, 45.22% random coil and 3.48% β-turn. Phylogenetic analysis showed that the StNF-Y sequence of potato was closely related to the NF-YC sequences of tomato, pepper and tobacco. In addition, StNF-Y played a certain role in a variety of plant stresses, so it was speculated that StNF-Y might be involved in the regulation of potato-related stress resistance. The results would provide a theoretical basis for further research on the function of potato StNF-Y and its resistance mechanism to stress.

Key words: Potato, StNF-Y, Bioinformatics, In silico cloning

CLC Number:

Q78
S532

SHI Li, WU Yaru, WANG Xiaojuan, CHANG Yannan, PANG Pengxiang, GAO Gang. Cloning and Bioinformatics Analysis of StNF-Y Nuclear Transcription Factor Gene in Potato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 31-37. doi: 10.7668/hbnxb.201751585.

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