Cloning and Sequence Analysis of Antheraea pernyi Nucleopolyhedrovirus (ApNPV) p11 Gene

doi:10.7668/hbnxb.2009.02.009

Abstract

Abstract: To enrich the molecular biology information of Antheraea pernyi nucleopolyhedrovirus( ApNPV),the p11 gene was PCR amplified and cloned. Moreover,the sequence was subjected to bioinformatics analysis. ApNPV p11 gene encoded a 102 amino acid sequence with a predicted molecular weight of 11.2 kDa.The position 1-33 of the N terminal was the signal peptide sequence and the middle position(50-72) was the transmembrane domain.PSI- BLAST search revealed that total 20 nucleopolyhedrovirus proteins significantly match with ApNPV P11 protein.Align analysis indicated that the middle parts was relatively conserved and the two ends were less conserved. Phylogenetic analysis classified ApNPV as one group I NPV and closely related to EppoNPV,AgMNPV and CfDefNPV.

Key words: Baculoviruses, p11 gene, Sequence analysis

CLC Number:

Q783

SHI Sheng-lin, LIU Yan-qun, PAN Min- hui, LU Cheng. Cloning and Sequence Analysis of Antheraea pernyi Nucleopolyhedrovirus (ApNPV) p11 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(2): 42-46. doi: 10.7668/hbnxb.2009.02.009.

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