Abstract: To elucidate the molecular characteristics of Sigma 1 gene of glutathione S-transferase sigma family,the gene named HvGSTs1 (GenBank:MF521977)was screened from the transcriptome of the adult of Heortia vitessoides. The gene was analyzed by bioinformatic approaches,and RT-qPCR was used to detect the relative expression of HvGSTs1 in various developmental stages and in different larval and adult tissues. The results showed that HvGSTs1 was 1 054 bp in length,encoding 204 amino acids. Sequence analysis showed that the amino acid sequences of HvGSTs1 protein contained two conserved domains,namely GST_N_Sigma_like and GST_C_Sigma_like. HvGSTs1 showed the highest homology with Chilo suppressalis (76%). Phylogenetic tree built by ClustalX and MEGA 4 indicated H.vitessoides with C.suppressalis were in the same branch. The relative expression levels of HvGSTs1 detected by RT-qPCR indicated that the expression levels of HvGSTs1 in pupae were higher than that in other stage of development. In larvae, HvGSTs1 had the highest expression level in fat body,nevertheless,the lowest in midgut. In adults, HvGSTs1 reached the highest expression level in abdomen and thorax and the expression level in foot was the lowest. The HvGSTs1 were prominently and differently expressed in all of the adult parts(P < 0.05). It lay the foundation for further discussion on physiological functions of Sigma family GST of H.vitessoides.

Key words: Heortia vitessoides, Glutathione S-transferase Sigma 1, Gene expression pattern, RT-qPCR

CLC Number: 

• Q966

• Q78