Abstract: This study laid the theoretical foundation for the study of the function and structure of peroxidase genes in tobacco and cloned the cDNA of peroxidase gene NtPOD1 from tobacco cultivar K326 by homologous cloning and analyzed the bioinformatics. At the same time,qRT-PCR method was used to analyze the expression pattern of tissue and organ of this gene and its stress response. The results showed that the total length of cDNA was 981 bp,encoding 326 amino acid residues,the predicted molecular weight was 37.19 ku,and the isoelectric point was 8.89. Bioinformatics analysis showed that the protein was a hydrophilic protein containing four conserved disulfide bonds and two conserved calcium binding sites in the domain,which may be located in extracellular(including cell wall).The class Ⅲ secretion oxidase of the dependent peroxidase superfamily had high homology with Nicotuana attenuata POD42, Nicotiana sylvestris POD42,Solanum tuberosum POD42 and so on. The gene was expressed in tobacco roots,stems,leaves and flowers,with the highest expression in leaves and the lowest expression in flowers. Meanwhile, NtPOD1 expression was induced by high salt,drought,hypokalemia,ABA and H₂O₂. The results indicate that NtPOD1 belongs to tobacco peroxidase and may play a role in the response of tobacco to abiotic stress.

Key words: Tobacco, Peroxidase, Gene cloning, Bioinformatics analysis, Expression pattern, Abiotic stress

CLC Number: 

• S572.03

• Q78