Abstract: In order to study response of the potassium concentration on the expression of KUP6 and KUP7 potassium transporter gene,hydroponic potato seedlings was conducted.The RNA of potato was isolated and checked, the first chain of the cDNA was synthesized by the Reverse Transcriptional Kit, and gene transcription on seedlings was detected by Real-time fluorescence quantitative PCR technique and the content of potassium in potato seedlings was detected.The content of potassium in potato seedlings was detected at 10, 20 and 30 days after potato seedling planting. The results showed that when potassium concentration was 1.00 mmol/L,the expression level of KUP6 and KUP7 was the highest. When potassium concentration was lower than 0.10 mmol/L,the expression was low,and the expression level of potassium decreased when potassium concentration was 10 mmol/L. It indicates that higher and lower potassium concentration is not conducive to the expression of KUP6 and KUP7 genes. Potassium content in roots, stems and leaves showed that potassium concentration reached its absorption saturation at 1.00 mmol/L for root. The higher potassium concentration in hydroponics, the higher potassium content in root, stem and leaf. The difference of potassium content in stem and leaf was decreased when lower potassium concentration in hydroponics and increased when higher concentration.

Key words: Potato, Potassium transporter, qPCR, KUP6, KUP7

CLC Number: 

• S141.3

• Q946